Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT288-25UL |
| Description | Anti-PICALM |
| Alternate Names |
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| Background Information | Phosphatidylinositol-binding clathrin assembly protein (UniProt: Q13492; also known as PICALM, Clathrin assembly lymphoid myeloid leukemia protein) is encoded by the PICALM (also known as CALM) gene (Gene ID: 8301) in human. PICALM is ubiquitously expressed protein that co-localizes with clathrin in the Golgi area. Five isoforms of PICALM have been reported that are produced by alternative splicing. PICALM acts as an assembly protein that recruits clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. It may also play a role in determining the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. It is reported to be involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. PICALM variants are associated with Alzheimers disease and are thought to be involved in the endocytosis and recycling of the amyloid protein precursor and it can affect Alzheimer s disease disk primarily by modulating production, transportation, and clearance of beta-amyloid peptides. (Ref.: Xu, W., et al. (2015). Mol Neurobiol. 52(1);399-413). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-PICALM Antibody, Cat. No. ABT288, is a rabbit polyclonal ntibody that detects PICALM in nhuman cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected PICALM in Jurkat cell lysate. Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PICALM in MOG-G-UVW human astrocytoma cell line (Courtesy of Dr Rhian Thomas from Cardiff University). Western Blotting Analysis: A 1:1,000 dilution from a representative lot PICALM in Mog-G-UVW human astrocytoma cell lysate and HeLa cell lysate (Courtesy of Dr Rhian Thomas from Cardiff University). Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PICALM in Human H4 neuroglioma cell lysate and SH-SY5Y neuroblastoma cell lysate (Courtesy of Dr Rhian Thomas from Cardiff University). Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected PICALM in human colon and human kidney tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Phosphatidylinositol-binding clathrin assembly protein (PICALM). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Phosphatidylinositol-binding clathrin assembly protein (PICALM) in human cells. it targets an epitope within 16 amino acids from the C-terminal half. It recognizes all 5 isoforms of PICALM. |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~61 kDa observed; 70.76, 68.76, 66.39, 59.92, and 70.04 kDa calculated, respectively for isoforms 1 to 5. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected PICALM in HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |