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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT79 |
| Description | Anti-WASH complex subunit FAM21C Antibody |
| Alternate Names |
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| Background Information | FAM21C, also known as WASH complex subunit FAM21C, is a part of the a 500 kDa core complex that allows the Wiskott-Aldrich syndrome protein (WASH) function. FAM21C can be found in the membranes of early endosomes. FAM21C cooperates with CAPZ and hinders its actin-capping activity, links WASH to endosomes, and is required for WASH-dependent retromer-mediated sorting. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-WASH complex subunit FAM21C Antibody detects level of WASH complex subunit FAM21C & has been published & validated for use in WB & IC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilutioin from a previous lot detected WASH complex subunit FAM21C in NIH/3T3, A431, and HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human WASH complex subunit FAM21C. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. In the complex, it probably mediates the recruitment of the complex to endosome membranes. SUBUNIT STRUCTURE: Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Interacts (via N-terminus) with WASH1; the interaction is direct. SUBCELLULAR LOCATION: Early endosome membrane MISCELLANEOUS: In human, FAM21 has undergone evolutionary duplication, with 4 highly homologous family members existing, including FAM21A, FAM21B, FAM21C, and an N-terminally truncated FAM21D form. SEQUENCE SIMILARITIES: Belongs to the FAM21 family. SEQUENCE CAUTION: The sequence AAH06456.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA25518.1 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | ~145 kDa observed Non-specific bands may be observed >200 kDa and at ~10 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HEK293 cell lysate. Western Blot Analysis: 5 µg/mL of this antibody detected WASH complex subunit FAM21C in 10 µg of HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |