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Chickens transfer high quantities of IgY (IgG) into the egg yolk and harvesting antibodies from eggs eliminates the need for the invasive bleeding procedure. One weeks eggs can contain 10 times more antibodies than the volume of rabbit blood obtained from one weekly bleeding. However, there are some disadvantages when using certain chicken derived antibodies in immunoassays. Chicken IgY does not fix mammalian complement component C1 and it does not perform as a precipitating antibody using standard solutions.
Product Information
Format
Purified
Presentation
Purified chicken polyclonal in buffer containing PBS with 0.05% sodium azide.
Quality Level
MQ100
Applications
Key Applications
Blocking of Intefering Antibodies
Application Notes
Gel Electrophoresis Analysis: 20 µg of reduced Chicken IgY from a representative lot was resolved by SDS-PAGE and visualized using total protein stain.
Note: To be used as a negative control for ChIP Ab+ MeCP2, Cat. No. 17-10491.
Note: For use as a negative control, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction.
Biological Information
Concentration
1 mg/mL
Host
Chicken
Isotype
IgY
Antibody Type
Polyclonal Antibody
Purification Method
T-gel
Molecular Weight
~180 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting using non-reduced Chicken IgY.
Western Blotting Analysis: 0.01 µg of this non-reduced Chicken IgY was detected by a Rabbit anti-Chicken secondary antibody.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
