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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, IHC, IH(P), FC | M | FITC | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL194F-25ASSAYS |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Cytokeratin 7, clone LP5K,FITCConj |
| Product Information | |
|---|---|
| Format | FITC |
| Presentation | The antibody is ammonium sulphate precipitation followed by ion exchange chromatography. The conjugate is supplied in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Cytokeratin 7 using this Anti-Cytokeratin 7 Antibody, clone LP5K, FITC conjugated validated for use in IC, IH, IH(P), FC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: Suitable for staining frozen sections and paraffin wax embedded tissue. Protease treatment of paraffin embedded sections is required prior to antibody application. Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003). Immunocytochemistry: isolated EVT cells fixed with 0.5% PFA at 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003). Flow cytometry: 10μL of conjugate per test. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Cytoskeletal proteins from cultured HeLa cells |
| Clone | LP5K |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | The antibody reacts with cytokeratin 7 which has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. FUSION PARTNER: Sp2/0 |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08729 # Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). SIZE: 469 amino acids; 51418 Da SUBUNIT: Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. PTM: Arg-20 is dimethylated, probably to asymmetric dimethylarginine. SIMILARITY: SwissProt: P08729 ## Belongs to the intermediate filament family. MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Tested |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year. |
| Packaging Information | |
|---|---|
| Material Size | 25 assays |