Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Mk, H | EM, FC, IF, FACS, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL496-I-25UL |
| Description | Anti-CD34 Antibody, clone QBEnd/10 |
| Alternate Names |
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| Background Information | Hematopoietic progenitor cell antigen CD34 (UniProt: P28906; also known as CD34) is encoded by the CD34 gene (Gene ID: 947) in human. CD34 is a highly glycosylated single-pass type I membrane protein that is expressed on hematopoietic progenitor cells and small vessel endothelium of a variety of tissues. Under normal conditions, CD34+ expressing cells account for about 1 2% of the total bone marrow cells. It serves as an adhesion molecule that plays a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It is also reported to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is synthesized with a signal peptide (aa 1-31) that is cleaved off in the mature form. The mature form has an extracellular domain (aa 32-290), a transmembrane domain (aa 291-311), and a cytoplasmic domain (aa 312-385). Two isoforms of CD34 have been described that are produced by alternative splicing. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-CD34, clone QBEnd/10, Cat. No. CBL496-I, is a mouse monoclonal antibody that detects CD34 and has been tested for use in Electron Microscopy, Flow Cytometry, Immunofluorescence and Fluorescence Activated Cell Sorting (FACS), Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD34 in human brain tissue sections. Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot was used to sort CD34+ cells from bone marrow. (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26). Immunofluorescence Analysis: A representative lot detected CD34 in Immunofluorescence applications (Miki, T., et. al. (2010). Mol Cancer Res. 8(5):665-76). Electron Microscopy Analysis: A representative lot detected CD34 in Electron Microscopy applications (Fina, L., et. al. (1990). Blood. 75(12):2417-26). Immunohistochemistry Analysis: A representative lot detected CD34 in Immunohistochemistry applications (Engler, J.R., et. al. (2012). PLoS One. 7(8):e43339; Fina, L., et. al. (1990). Blood. 75(12):2417-26). Flow Cytometry Analysis: A representative lot detected CD34 in Flow Cytometry applications (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26; Fina, L., et. al. (1990). Blood. 75(12):2417-26). Western Blotting Analysis: A representative lot detected CD34 in Western Blotting applications (Fina, L., et. al. (1990). Blood. 75(12):2417-26). |
| Biological Information | |
|---|---|
| Immunogen | Human placental endothelial membrane vesicles. |
| Clone | QBEnd/10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone QBEnd/10 specifically detects CD34 in human and non-human primates. |
| Isotype | IgG1λ |
| Species Reactivity |
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| Species Reactivity Note | Human, Monkey. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 40.72 kDa Calculated. This antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected CD34 in human kidney tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |