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Millipore Detect Mouse Igg1 Using This Milli-Mark Anti-Mouse Igg1-Fitc Antibody, Clone Mopc-21 Validated For Use In Fc. - Mill (Additional S&H or Hazmat Fees May Apply)
The MOPC-21 tumor line that produces mouse IgG1,k, is a mineral oil induced plasmacytoma. The product is originated and carried intraperitoneally in BALB/c mice. Identity and purity of the immunoglobulin is established by immunoelectrophoresis (IEP) and Ouchterlony Double Diffusion (ODD), prior to conjugation. Electrophoresis of the purified immunoglobulin, followed by diffusion versus anti-mouse whole serum, antimouse IgG1 and anti-mouse kappa chain, results in single arcs of precipitation. By ODD the purified immunoglobulin preparation is non-reactive with antisera to mouse lambda light chain, mouse IgA, IgM, IgG2a, IgG2b, and IgG3. The specificity of staining by monoclonal antibodies to human CD antigens should be verified by establishing the amount of non-specific binding to the target cell population. It is recommended that a non-reactive immunoglobulin of the same isotype, concentration and F/P molar ratio be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.
Product Information
Format
FITC
Presentation
Purified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality Level
MQ100
Applications
Application
Detect mouse IgG1 using this Milli-Mark Anti-mouse IgG1-FITC Antibody, clone MOPC-21 validated for use in FC.
Key Applications
Flow Cytometry
Biological Information
Clone
MOPC-21
Host
Mouse
Specificity
This mouse IgG1 isotype control antibody is not reactive with any known antigen.
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Purification Method
Immunoelectrophoresis
Product Usage Statements
Quality Assurance
Evaluated for use as flow cytometry isotype control using Jurkat Cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
