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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Rb | AlexaFluor®647 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | FCMAB288A6 |
| Trade Name |
|
| Description | Milli-Mark™ Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3 |
| Background Information | Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis. |
| Product Information | |
|---|---|
| Format | AlexaFluor®647 |
| Control |
|
| Presentation | Purified recombinant rabbit monoclonal IgG1 conjugated to Alexa Fluor 647 in PBS with 0.1% sodium azide and 15 mg/mL BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Milli-Mark Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3 is validated for use in FC for the detection of Ubiquitin. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Immunogen used was di-ubiquitin linked at Lys63. |
| Epitope | Lys63 ubiquitination site |
| Clone | Apu3 |
| Host | Rabbit |
| Specificity | Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ref.21 SUBCELLULAR LOCATION: Cytoplasm. Nucleus. PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly. MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a). SIMILARITY: Belongs to the ubiquitin family. |
| Molecular Weight | Molecular weight varies. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by flow cytometry using HeLa cells |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 tests |