Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IHC, FC, IP | AHm | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1399Z-I-100UG |
| Description | Anti-Integrin αX Antibody, clone N418 Antibody, azide free |
| Alternate Names |
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| Background Information | Integrin alpha-X (UniProt: Q9QXH4; also known as CD11 antigen-like family member C, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95, CD11c) is encoded by the Itgax gene (gene ID: 16411) in murine species. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD11c is a single-pass type I membrane protein. It has an extracellular domain (aa 20-1116), a transmembrane domain (aa 1117-1137), and a cytoplasmic domain (aa 1138-1169). It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Low levels of CD11c are expressed on human granulocytes and monocytes, and higher expression levels are observed on macrophages, dendritic cells and hairly cell leukemias. CD11c associates with beta-2 subunit in a non-covalent manner and then serves as a receptor for fibrinogen where it recognizes the sequence g-P-R. CD11c is shown to mediate cell-cell interaction during inflammatory responses and is important in monocyte adhesion and chemotaxis. Clone N418 is known to recognize an epitope on the p150 alpha chain. (Ref.: Metlay, JP et Al. (1990). J. Exp. Med. 171(5); 1753-1771). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified armenian hamster monoclonal antibody IgG in PBS without preservatives. |
| Applications | |
|---|---|
| Application | Anti-Integrin X, clone N418, azide free, Cat. No. MAB1399Z-I, is an armenian hamster monoclonal antibody that detects Integrin alpha-X and has been tested for use in Immunohistochemistry, Flow Cytometry, and Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot detected Integrin X in Immunoprecipitation applications (Metlay, J.P., et. Al. (1990). J Exp Med. 171(5):1753-71). Immunohistochemistry Analysis: A representative lot detected Integrin X in Immunohistochemistry applications (Tan, C.S.E., et. Al. (2017). PLoS Pathog. 13(4):e1006311; Roland, C.L., et. Al. (2009). Mol Cancer Ther. 8(7):1761-71; Metlay, J.P., et. Al. (1990). J Exp Med. 171(5):1753-71). Flow Cytometry Analysis: A representative lot detected Integrin X in Flow Cytometry applications (Metlay, J.P., et. Al. (1990). J Exp Med. 171(5):1753-71; Granucci, F., et. Al. (1997). J Immunol. 159(4):1794-99; Cervantes-Barragan, L., et. Al. (2007). Blood. 109(3):1131-37). |
| Biological Information | |
|---|---|
| Immunogen | Mouse spleen dendritic cells. |
| Clone | N418 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Armenian Hamster |
| Specificity | Clone N418 is a mouse monoclonal antibody that detects integrin alpha-X in murine tissue. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 129.15 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in mouse spleen frozen tissue sections. Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected Integrin X in mouse spleen frozen tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |