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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC, IP, FC, IF, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1680-C |
| Description | Anti-Filamin A Antibody, clone TI10, Ascites Free |
| Alternate Names |
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| Background Information | Filamin is a structural protein that forms flexible cross-links between two actin filaments. Filamin is a homodimer of polypeptide chains each joined to the other at one end with an actin binding site ath the other. It is present in smooth muscle, fibroblasts, platelets and lymphocytes. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Filamin A Antibody, clone TI10, Ascites Free is validated for use in western blotting, IHC, IP, flow cytometry, immunofluorescence & ICC for the detection of Filamin A. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 and 1:500 dilution from a representative lot detected Filamin A in human prostate and kidney tissues, respectively. Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Filamin A in human tumor tissue sections (Bedolla, R. g., et Al. (2009). Clin Cancer Research. 15(3):788-796.). Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in human normal and tumor tissue lysates (Bedolla, R. g., et Al. (2009). Clin Cancer Research. 15(3):788-796.). Immunoprecipitation/Western Blotting Analysis: A representative lot from an independent laboratory immunoprecipitated Filamin A from transfected HEK293T cell lysates. The immunoprecipitated sample was then subjected to Western Blotting using the same lot of antibody (Nakagawa, K., et Al. (2010). Biochem. J. 427(2):237–245.). Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in transfected U937 cell lysate (Beekman, J. M., et Al. (2008). J Immunol. 180(6):3938-3945.). Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in prostate cancer cell lysate. (Lin, J., et Al. (2007). Int. J. Cancer. 121(12):2596–2605.). Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in LNCap and C4-2 whole cell lysates (Wang, Y., et Al. (2007). Oncogene. 26(41):6061–6070.). Western Blotting Analysis: A representative lot from an independent laboratory detected pulled-down human Filamin A proteins. (Lad, Y., et Al. (2007). EMBO J. 26(17):3993–4004.). Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in lysate samples from normal bladder mucosa and muscle invasive bladder tumor (Smith, S. C., et Al. (2007). Clin Cancer Res. 13(13):3803-3813.). Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in lysate samples from human prefontal cortical tissue (KOH, P. O., et Al. (2003). Ach Gen Psychiatry. 60(3):311-319.). Flow Cytometry Analysis: A representative lot from an independent laboratory detected Filamin A in transfected U937 cells (Beekman, J. M., et Al. (2008). J Immunol. 180(6):3938-3945.). Flow Cytometry Analysis: A representative lot from an independent laboratory detected Filamin A in LAN-1 cells (Bachmann, A. S., et Al. (2006) Cancer Sci. 97(12):1359–1365.). Immunofluorescence Analysis: A representative lot from an independent laboratory detected Filamin A in clinical patient samples (Kley, R. A., et Al. (2007). Brain. 130(Pt 12):3250-3264.). Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Filamin A in LAN-1 cells (Bachmann, A. S., et Al. (2006) Cancer Sci. 97(12):1359–1365.). |
| Biological Information | |
|---|---|
| Immunogen | Human platelet protein corresponding to human Filamin A. |
| Clone | TI10 |
| Concentration | 1 mg/mL |
| Host | Mouse |
| Specificity | This antibody recognizes Filamin A (actin-binding protein), both the unprocessed form (270-280 kDa), and the C-terminal (90-100 kDa) calpain cleavage fragment of Filamin A (Aakhus, 1992). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Bovine based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | ~110 kDa observed. Phosphorylation of this protein may cause the band to shift in Western Blotting. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human uterus tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Filamin A in 10 µg of human uterus tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |