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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, ICC, IP, IHC, ELISA, IF | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2148-C |
| Replaces | 04-1074, MAB2148 |
| Description | Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free) |
| Alternate Names |
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| Background Information | PECAM-1 is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free) is validated for use in flow cytometry, ICC, IP, IHC, ELISA & immunofluorescence for the detection of PECAM-1. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in pluripotent cells (Boyd, N. L., et Al. (2007). Exp Biol Med. 232(6):833-843.). Immunoprecipitation Analysis: A representative lot detected PECAM-1 by immunoprecipitation. Immunohistochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et Al. (2005). Cancer Res. 65(24):11419-28.). ELISA Analysis: A representative lot of from an independent laboratory detected PECAM-1 in a panel of CD31 mutant cell lines (Newton, J. P, et Al. (1997) J. Biol. Chem., 272: 20555-63.). Immunofluorescence Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et Al. (2005). Cancer Res. 65(24):11419-28.). |
| Biological Information | |
|---|---|
| Immunogen | Whole cells expressing human PECAM-1. |
| Clone | P2B1 |
| Concentration | 1 mg/mL |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | ~80 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in human PBMCs. Flow Cytometry Analysis: 1 µg of this antibody detected PECAM-1 in 1X10E6 human PBMCs. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |