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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, H, M, Po, R, Rb | ICC, IHC, IH(P), WB | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB360-25UL |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 (mouse monoclonal) |
| Alternate Names |
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| Background Information | Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Ascites fluid. Liquid. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: A previous lot was used on cultured mammalian cells. Immunohistochemistry(paraffin): 1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain. Immunoblotting: A 1:1000 dilution of a previous lot was used. Optimal working dilutions must be determined by the end user |
| Biological Information | |
|---|---|
| Immunogen | Purified GFAP from porcine spinal cord. |
| Clone | GA5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Reacts with Bovine, Chicken, Human, Mouse, Porcine (Pig), Rabbit and Rat. Reactivity with other species has not been determined. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. SIZE: 432 amino acids; 49880 Da SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1. SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments. DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family. |
| Molecular Weight | ~ 51 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on Mouse brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |