Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IF, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3839-I-100UG |
| Replaces | MAB3839 |
| Description | Anti-Transglutaminase-2 Antibody, FN binding domain Antibody, clone 4G3 |
| Alternate Names |
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| Background Information | Protein-glutamine gamma-glutamyltransferase 2 (UniProt: P21980; also known as EC: 2.3.2.13, Tissue transglutaminase, Transglutaminase C, TG(C), TGC, TGase C, Transglutaminase H, TGase H, Transglutaminase-2, TGase-2, TG2) is encoded by D13the TGM2 gene (Gene ID: 7052) in human. TG2 is a multi-domain, multi-functional enzyme that is shown to post-translationally modify proteins by catalyzing the formation of intermolecular isopeptide bonds between glutamine and lysine side-chains. It catalyes the calcium-dependent covalent modification of protein-bound glutamine side chains through transamidation or deamidation reactions. Recycling endosomes is reported to be essential for externalization of TG2, which does not follow the classical ER/Golgi-dependent secretion pathway, but is targeted to perinuclear recycling endosomes, and is delivered inside these vesicles prior to externalization. Inactivation of recycling endosomes or blocking endosome fusion with the plasma membrane is shown to block TG2 secretion. TG2 plays a critical role in extracellular matrix formation and integrin mediated signaling. It is also reported to be involved in cell signaling involving 7-transmembrane receptors. It also participates in the pathogenesis of neurodegenerative diseases and certain cancers. (Ref.: Zemskov, EA., et Al (2011). PLoS ONE 6(4): e19414; Siegal, M., and Khosla, C. (2007). Pharmacol. Ther. 115(2); 232-245. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without azide. |
| Applications | |
|---|---|
| Application | Anti-Transglutaminase-2, FN binding domain, clone 4G3, Cat. No. MAB3839-I, is a mouse monoclonal antibody that detects Protein-glutamine gamma-glutamyltransferase 2 and has been tested for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 4 µg/mL from a representative lot detected Transglutaminase-2, FN binding domain in HUVEC lysate. Western Blotting Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Western Blotting applications (Janiak, A., et. Al. (2006). Mol Biol Cell. 17(4):1606-19). Radioimmunoassay Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Radioimmunoassay applications (Akimov, S.S., et. Al. (2001). Blood. 98(5):1567-76). Flow Cytometry Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Flow Cytometry applications (Zemskov, E.A., et. Al. (2011). PLos One. 6(4):e19414). Immunoprecipitation Analysis: A representative lot immunoprecipitated Transglutaminase-2, FN binding domain in Immunoprecipitation applications (Zemskov, E.A., et. Al. (2011). PLos One. 6(4):e19414; Janiak, A., et. Al. (2006). Mol Biol Cell. 17(4):1606-19). Immunofluorescence Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Immunofluorescence applications (Janiak, A., et. Al. (2006). Mol Biol Cell. 17(4):1606-19; Akimov, S.S., et. Al. (2001). Blood. 98(5):1567-76; Zemskov, E.A., et. Al. (2011). PLos One. 6(4):e19414). |
| Biological Information | |
|---|---|
| Immunogen | A full-length purified tissue transglutaminase (TG-2) from human erythrocytes. |
| Clone | 4G3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4G3 specifically detects human Transglutaminase-2. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~80 kDa observed; 77.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Transglutaminase-2, FN binding domain in A431 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |