Special Offers
100% Performance Guaranteed
| Description | |
|---|---|
| Catalogue Number | MAB4190-25UG |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Ki-67, clone Ki-S5 |
| Background Information | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkins lymphoma (Kreipe et al., 1993), mammary carcinomas (Mauri et al., 1984), ovarian tumors (Ballin et al., 1994) and prostate cancer. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Ki-67 Antibody, clone Ki-S5 is a high quality Mouse Monoclonal Antibody for the detection of Ki-67 & has been validated in FC, ICC, IHC, IHC(P) & WB. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: A 5-10 μg/mL concentration of a previous lot was used in IC. Immunohistochemistry: A 5-10 μg/mL concentration of a previous lot was used in IH. Immunohistochemistry: 5-10 µg/mL on formalin fixed paraffin tissue. Citrate buffer-microwave antigen retrieval Flow Cytometry: A previous lot of this antibody was used in FC. Western blot: 1-10 µg/mL Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Nuclear protein preparation from the human cell line U937.50. |
| Clone | Ki-S5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | In Immunoprecipitation and Western blot experiments the Ki-S5 antibody recognizes a cell-cycle-associated protein of 345 kD and 395 kD (Kreipe et al., 1993) identical with the Ki-67 antigen. The antibody binds to a formalin-resistant epitope of the Ki-67 antigen. The immunoreactivity of Ki-S5 is confined to the nuclei of proliferating cells and no crossreactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval (Mauri et al., 1984). A comparison of immunohistochemical labeling of fresh and fixed tissue samples of NHL showed that identical results were obtained with Ki-67 and Ki-S5 (Kreipe et al., 1993). The Ki-67 antigen is preferentially expressed during late G1, S, G2 and M phase of the cell cycle, while resting, non-cycling cells (G0 phase) lack the Ki-67 antigen. In addition, constantly proliferating cells (e.g. cell lines) react positively to Ki-S5 during the entire cell-cycle. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkins lymphoma (Kreipe et al., 1993), mammary carcinomas (Mauri et al., 1984), ovarian tumors (Ballin et al., 1994) and prostate cancer. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P46013 # Thought to be required for maintaining cell proliferation. SIZE: 3256 amino acids; 358694 Da SUBUNIT: Binds through the FHA domain to MKI67IP. SUBCELLULAR LOCATION: Nucleus. Note=Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes. DEVELOPMENTAL STAGE: Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. SIMILARITY: SwissProt: P46013 ## Contains 1 FHA domain. |
| Molecular Weight | 345 & 395 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on A431 lysates. Western Blot Analysis: 1:500 dilution of this antibody detected cell-cycle-associated protein of 345 kDa and 395 kDa on 10 μg of A431 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |