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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | FC, IF, IHC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4301-25UG |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-SSEA-1, clone MC-480 |
| Alternate Names |
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| Background Information | SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide mouse monoclonal IgM in buffer containing 0.05M |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: A previous lot of this antibody was tested on IH. Immunoprecipitation: A previous lot of this antibody was tested on IP. Immunofluorescence: A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external. Flow Cytometry: A starting range of 10-20 µg/mL is suggested. Positive mouse EC lines include: F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25. Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | F9 tetracarcinoma stem cells (X-irradiated). |
| Clone | MC-480 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). |
| Isotype | IgM |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | FUT4 catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). It has been detected in human embryos (5-10 weeks) suggesting a role in development. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P22083 # May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. SIZE: 405 amino acids; 45570 Da SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. SIMILARITY: SwissProt: P22083 ## Belongs to the glycosyltransferase 10 family. |
| Stem Cell Type |
|
| Molecular Weight | 220 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Tested |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |