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| Description | |
|---|---|
| Catalogue Number | MAB5326-25UG |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Ms X Nestin Antibody, clone 10C2 Human Specific |
| Background Information | Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Nestin Antibody, clone 10C2 is validated for use in IC, IH, IH(P), WB for the detection of Nestin. |
| Key Applications |
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| Application Notes | Immunoblotting: 1:1,000-1:2,000 on denatured protein. Western blot: 1:1000-1:5000. Recognizes a 220-240kDA band on denaturing SDS gels. There is also a 60-64kDa band present in most lysates, the identity of this smaller fragment is unknown but it is not seen in lysates not containing human nestin protein. WI-38 (ATCC# CCL75) can be used as a positive control. U251 cell line is also positive. Immunocytochemistry: 1:200 on cells fixed in 4% paraformaldehyde and permeabilized with 0.02% Triton Immunohistochemistry: paraformaldehyde is the preferred fixative: 1:200 on formalin fixed, paraffin embedded tissue sections. Use triton 0.02% in block only; dilution buffer 0.1% BSA; incubations 1-2 hours RT. Human fetal brain is suggested as a positive control. (CA Messam et al, Develop. Brain Res, 134:87-92, 2002; Gu, et al., Brain Res. 943:174-180, 2002). Filamentous staining in the cytoplasm of cells Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Fusion protein. |
| Epitope | human |
| Clone | 10C2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Reacts with human Nestin, with weak reactivity to monkey nestin. Not reactive with rodent nestin. |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM] |
| Gene Symbol |
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| Non-Reactive Species |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P48681 # May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division (By similarity). SIZE: 1621 amino acids; 177439 Da SUBUNIT: Interacts with FHOD3 (By similarity). TISSUE SPECIFICITY: CNS stem cells. DEVELOPMENTAL STAGE: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. SIMILARITY: SwissProt: P48681 ## Belongs to the intermediate filament family. |
| Stem Cell Type |
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| Molecular Weight | 200-220 kDa |
| Product Usage Statements | |
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| Quality Assurance | Tested |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Antibodies may be stored at 4°C or -20°C. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the containers cap. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |