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| Description | |
|---|---|
| Catalogue Number | MAB5460-25UL |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | MOUSE ANTI-NKX2.1 MONOCLONAL ANTIBODY |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Ascites fluid. Liquid. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Nkx2.1 Antibody is validated for use in WB, IH(P) for the detection of Nkx2.1. |
| Key Applications |
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| Application Notes | Western blot. The antibody reacts with a 40 kDa band in immunoblots of nuclear extracts or whole cell lysates from the Nkx2.1 positive cell lines MLE15, H441-4, H345 and rat type II pmeumocyte cells. Immunohistochemistry. The antibody can be used on formalin-fixed paraffin embedded tissue. Heat induced epitope retrieval is recommended. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant rat Nkx2.1. |
| Clone | 8G7-G3-1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Nkx2.1 (Thyroid Transcription Factor-1, TTF-1). |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P43699 # Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. SIZE: 371 amino acids; 38596 Da SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Thyroid and lung. PTM: Phosphorylated on serine residues (By similarity). DISEASE: SwissProt: P43699 # Defects in TITF1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances. & Defects in TITF1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems. SIMILARITY: SwissProt: P43699 ## Belongs to the NK-2 homeobox family. & Contains 1 homeobox DNA-binding domain. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Tested |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
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| Material Size | 25 µL |