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Millipore Detect Bcl2l12 Using This Mouse Monoclonal Anti-Bcl2l12, Clone 11b5.1 Antibody, Cat. No. Mabc1022, Validated For Use In Immunohistochemistry (Paraffin) And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Bcl-2-like protein 12 (UniProt Q9HB09; also known as Bcl-2-related proline-rich protein, Bcl2-L-12) is encoded by the BCL2L12 (also known as BPR) gene (Gene ID 83596) in human. Bcl-2 family proteins paly either a pro- or anti-apoptotic role in cell death signaling. BCL-2 family members are related by regions of sequence and structural homology. The pro-survival members contain four BCL-2 homology regions (BH1 BH4), whereas the BH3-only members contain only the BH3 amphipathic helix that mediates their interaction with the groove of multi-domain BCL-2 family members. Bcl2-L-12 is an anti-apoptotic protein that shares a lower amino acid similarity with the other Bcl2 family members. It contains a BH2 (a.a. 311-322) domain and a BH3-like domain (a.a. 192-220) that mediate its interaction with Bcl2 and Bcl-xL. Exogenous expression of Bcl2-L-12 with Leu213 or Leu217 mutation in U87MG cell line is reported to result in reactivation of PARP cleavage, caspase-3 and cytochrome c release compared to cells expressing wild-type Bcl2-L-12.
Product Information
Format
Purified
Presentation
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Bcl2L12 using this mouse monoclonal Anti-BCL2L12, clone 11B5.1 Antibody, Cat. No. MABC1022, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BCL2L12 in human pancreas, skeletal muscle, and tonsil tissue sections.
Biological Information
Immunogen
GST-tagged recombinant human BCL2L12 internal fragment.
Clone
11B5.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 11B5.1 targets an internal epitope present in spliced isoforms 1&3, but absent in isoform 2, of human BCL2L12 reported by UniProt (Q9HB09).
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_619580
Gene Symbol
BCL2L12
BPR
Purification Method
Protein G purified.
UniProt Number
Q9HB09
Molecular Weight
~37 kDa observed. 36.82/36.67 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in PC3 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected BCL2L12 in 10 µg of PC3 human prostate cancer cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
