Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB, IF, IH(P), IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1108-100UG |
| Description | Anti-LAMP-1 Antibody, clone H4A3 |
| Alternate Names |
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| Background Information | Lysosome-associated membrane glycoprotein 1 (UniProt: P11279; also known as LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a) is encoded by the LAMP1 gene (Gene ID: 3916) in human. LAMP-1 and LAMP-2 are two highly glycosylated proteins found in the lysosomal membrane. They may contribute up to 50% of all proteins present in the lysosomal membranes. They are known to maintain the lysosomal acidification and lysosomal membrane integrity. Extensive N-glycosylation on LAMP-1 and 2 to protects them from the lysosomal proteolysis. However, higher levels of poly-N-acetyllactosamines on LAMP-1 and 2 have been correlated with decreased cellular differentiation and enhanced metastatic potential. LAMP-1 is a single-pass type I transmembrane protein that contains a large lumenal domain (aa 29-382), a transmembrane domain (aa 383-405), and a short C-terminal cytoplasmic tail (aa 406-417). LAMP-1 is synthesized with a signal peptide (aa 1-28) that is subsequently cleaved in the mature form. LAMP-1 presents carbohydrate ligands to selectins and is also reported to act as a receptor for Lassa virus protein. Two isoforms of LAMP-1 have been described that are produced by alternative splicing. (Ref.: Tan, K-P., et Al. (2016). Cell Death Dis. 7, e2347). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-LAMP-1, clone H4A3, Cat. No. MABC1108, is a mouse monoclonal antibody that detects Lysosome-associated membrane glycoprotein 1 (LAMP-1) and has been tested for use in DOT Blot, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected LAMP-1 in HeLa and A431 cells. Immunohistochemistry (Paraffin) Analysis: A representative lot detected LAMP-1 in Immunohistochemistry applications (Furuta, K., et. Al. (2001). Am J Pathol. 159(2):449-55). Western Blotting Analysis: A representative lot detected LAMP-1 in Western Blotting applications (Mane, S.M., et. Al. (1989). Arch Biochem Biophys. 268(1):360-78). Immunofluorescence Analysis: A representative lot detected LAMP-1 in Immunofluorescence applications (Starr, T., et. Al. (2011). Cell Host Microbe. 11(1):33-45). DOT Blot Analysis: A representative lot detected LAMP-1 in DOT Blot applications (Arruda, L.B., et. Al. (2006). J Immunol. 177(4):2265-75). Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected LAMP-1 in human kidney and human pancreas tissue sections. Immunoprecipitation Analysis: A representative lot immunoprecipitated LAMP-1 in Immunoprecipitation applications (Mane, S.M., et. Al. (1989). Arch Biochem Biophys. 268(1):360-78). |
| Biological Information | |
|---|---|
| Immunogen | Human adherent peripheral blood cells. |
| Clone | H4A3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone H4A3 detects Lysosome-associated membrane glycoprotein 1 (LAMP-1) in human cells. |
| Isotype | IgGκ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~110 kDa observed; 44.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected LAMP-1 in HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |