Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Rb | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1127-25UL |
| Description | Anti-FGFR-4 Antibody, clone 29.2 |
| Alternate Names |
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| Background Information | Fibroblast growth factor receptor 4 (UniProt: P22455; also known as EC: 2.7.10.1, FGFR-4, CD334) is encoded by the FGRF4 (also known as JTK2, TKF) gene (Gene ID: 2264) in human. FGFR-4 is a highly conserved, single-pass type I membrane protein with tyrosine kinase activity that serves as a cell surface receptor for fibroblast growth factors and regulates cell proliferation differentiation, and migration. FGFR-4 is expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. In the absence of a ligand, it is present as a monomer in an inactive conformation. Upon ligand binding it homodimerizes into an active conformation with autophosphorylation of tyrosine residues. FGFR-4 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It contains an extracellular domain (aa 22-369), a transmembrane domain (aa 370-390), and a cytoplasmic domain (aa 391-802). FGFR-4 undergoes N-glycosylation, and when not fully glycosylated it can undergo proteasomal degradation. FGFR-4 signaling is down-regulated by receptor internalization and degradation. MMP14 is reported to promote internalization and degradation of FGFR-4. Mutations that lead to constitutive kinase activation or impair normal FGFR-4 inactivation lead to aberrant signaling leading to tumor growth. (Ref.: Turkington, RC et Al. (2014). Cell Death Dis. 5; e1046). |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Presentation | Rabbit monoclonal antibody in supernatant without preservatives. |
| Applications | |
|---|---|
| Application | Anti-FGFR-4, clone 29.2, Cat. No. MABC1127, is a rabbit monoclonal antibody that detects Fibroblast growth factor receptor 4 and has been tested for use in Flow Cytometry. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A 1:4 dilution from a representative lot detected FGFR-4 in one million LNCaP cells. |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant extracellular domain of human fibroblast growth factor receptor 4 (FGFR-4). |
| Epitope | extracellular domain |
| Clone | 29.2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit monoclonal antibody detects human fibroblast growth factor receptor 4 (FGFR-4). It targets an epitope with in the extracellular domain. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | 87.95 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in DU-145 cells. Flow Cytometry Analysis: A 1:4 dilution of this antibody detected FGFR-4 in one million DU-145 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |