Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IH(P), INHIB | AHm | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1131-25UG |
| Description | Anti-PD-L1 B7-H1 Antibody, clone 10B5 |
| Alternate Names |
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| Background Information | Programmed cell death 1 ligand 1 (UniProt: Q9EP73; also known as PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274) is encoded by the Cd274 (also known as B7h1, Pdcd1l1, Pdcd1lg1, Pdl1) gene (Gene ID: 50533) in murine species. PD-L1 is a single-pass type I membrane protein that is highly expressed in the heart, thymus, skeletal muscle, and lung. Its expression is also reported on activated dendritic cells, B-cells, and macrophages. Numerous tumor cell lines of lymphoid origin also express PD-L1. PD-L1 expression can be up-regulated by interferon-gamma treatment in monocytes, B cells, and macrophages. PD-1 and its ligands, PD-L1 and PD-L2 play a key role in the maintenance of peripheral tolerance, a process by which the quiescence of autoreactive mature T cells is maintained. However, tumors and pathogens that cause chronic infections can exploit this pathway to escape T-cell mediated tumor-specific and pathogen-specific immunity. The effector functions of T-cells expressing PD-1 can be downregulated by PD-L1 or PD-L2 expressed by the tumor cells. PD-L1 is initially produced with signal peptide (aa. 1-18) sequence, the removal of which yields the mature protein with a large extracellular (aa. 19-239) domain, a transmembrane domain (aa 240-260), and a cytoplasmic domain (aa 261-290). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified armenian hamster monoclonal antibody IgG2-IgG3 in PBS without azide. |
| Applications | |
|---|---|
| Application | Anti-PD-L1 B7-H1, clone 10B5, Cat. No. MABC1131, is an Armenian hamster monoclonal antibody that detects Programmed cell death 1 ligand 1 (PD-L1) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Inhibition Function assay. |
| Key Applications |
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| Application Notes | Inhibits Activity/Function Analysis: A representative lot blocked the activity of PD-L1 B7-H1 and inhibited apoptosis of activated alloreactive 2C T cells. (Hirano, F., et. Al. (2005). Cancer Res. 65(3):1089-96; Goldberg, M.V., et. Al. (2007). Blood. 110(1):186-92; Joshi, T., et. Al. (2009). PLoS Pathog. 5(5):e1000431; Liu, X., et. Al. (2016). Sci Rep. 6:36722). Flow Cytometry Analysis: A representative lot detected PD-L1 B7-H1 in Flow Cytometry applications (Hirano, F., et. Al. (2005). Cancer Res. 65(3):1089-96; Dong, H., et. Al. (2004). Immunity. 20(3):327-36). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant fragment corresponding to the extracellular domain of Mouse Programmed cell death 1 ligand 1. |
| Epitope | extracellular domain |
| Clone | 10B5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Armenian Hamster |
| Specificity | This Armenian hamster monoclonal antibody detects Programmed cell death 1 ligand 1 (PD-L1) in murine species. |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 32.78 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in mouse heart tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected PD-L1 B7-H1 in mouse heart tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |