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Millipore Detects Raf Kinase Inhibitor Protein Using This Mouse Monoclonal Anti-Rkip, Clone 7f12, Cat. No. Mabc1136, Tested For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Phosphatidylethanolamine-binding protein 1 (UniProt: P30086; also known as PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP) is encoded by the PEBP1 (also known as PBP, PEBP) gene (Gene ID: 5037) in human. RKIP is as member of the phosphatidylethanolamine-binding-protein (PEBP) family that antagonizes multiple cell-survival pathways, such as the Ras-Raf-1, MEK/ERK, NF- B, and G-protein-coupled receptor Kinase 2 and thereby modulates cell growth, apoptosis, motility, invasion and metastasis. Over-expression of RKIP is shown to inhibit c-Src auto-phosphorylation and activation and IL-6-, JAK1 and 2-, and Raf-mediated STAT3 phosphorylation and activation. RKIP overexpression is shown to sensitize tumor cells to chemotherapeutic drug-induced apoptosis. Hence, it is considered as a suppressor of metastasis and a prognostic marker in several cancers. Clone 7F12 specifically detects denatured RKIP endogenously expressed in 293 cells and several pancreatic cell lines. (Ref.: Odabaei, G., et al. (2004). Adv. Cancer Res. 91; 169-200; Yousuf, S., et al. (2014). PLoS One 9(3); e92478).
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Applications
Application
Detects Raf kinase inhibitor protein using this mouse monoclonal Anti-RKIP, clone 7F12, Cat. No. MABC1136, tested for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected RKIP in Western Blotting applications (Wang, X., et. al. (2010). J Immunol Methods. 362(1-2):151-60).
Biological Information
Immunogen
Purified native and denatured Raf kinase inhibitor protein (RKIP).
Clone
7F12
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 7F12 specifically detects human Raf kinase inhibitor protein, (RKIP).
Isotype
IgG1κ
Species Reactivity
Human
Species Reactivity Note
Human.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002558.1
Gene Symbol
PEBP1
PBP
PEBP
Purification Method
Protein G purified
UniProt Number
P30086
Molecular Weight
~23 kDa; 21.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HEK293 cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected RKIP in HEK293 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
