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Millipore Detects Hippocalcin-Like Protein 1 Using This Mouse Monoclonal Anti-Hpcal1, Clone 5a5, Cat. No. Mabc1138, Tested For Use In Western Blotting, Immunohistochemistry (Paraffin), - Mill (Additional S&H or Hazmat Fees May Apply)
Hippocalcin-like protein 1 (UniProt: P37235; also known as Calcium-binding protein BDR-1, HLP2, Visinin-like protein 3, VILIP-3) is encoded by the HPCAL1 (also known a BDR1) gene (Gene ID: 3241) in human. HPCAL1 is a lipid-anchor membrane protein of the visinin-like subfamily of neural calcium sensor and is involved in the calcium-dependent regulation of rhodopsin phosphorylation. It is structurally related to Ca2+-myristoyl switch proteins that contain four EF-hand motifs and a covalently attached N-terminal myristoyl group. HPCAL1 is also expressed in Purkinje cells of the cerebellum and hippocampus where it is believed to regulate synaptic plasticity relevant for learning and memory. Clone 5A5 can detect HPCAL1 in formalin-fixed 293 cells and in pancreatic cell lines. (Ref.: Chen, Y., et al. (2014). Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 33(1); 20-27; Li, C., et al. (2016). PLoS One 11(11); e0165921).
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Applications
Application
Detects Hippocalcin-like protein 1 using this mouse monoclonal Anti-HPCAL1, clone 5A5, Cat. No. MABC1138, tested for use in Western Blotting, Immunohistochemistry (Paraffin), ELISA and Immunocytochemistry.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
ELISA
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A representative lot detected HPCAL1 in Immunocytochemistry applications (Chen, Y., et. al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7).
ELISA Analysis: A representative lot detected HPCAL1 in ELISA applications (Chen, Y., et. al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7).
Immunohistochemistry (Paraffin) Analysis: A 1:50-250 Dilution from a representative lot detected HPCAL1 in human kidney and human cerebellum tissue sections.
Biological Information
Immunogen
Native and denatured human HPCAL1.
Clone
5A5
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 5A5 specifically detects Hippocalcin-like protein 1 (HPCAL1) in human cells.
Isotype
IgG1κ
Species Reactivity
Human
Species Reactivity Note
Human.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_602293.1
Gene Symbol
HPCAL1
BDR1
Purification Method
Protein G purified
UniProt Number
P37235
Molecular Weight
~22 kDa observed; 22.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Panc-1 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected HPCAL1 in Panc-1 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
