Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P), ELISA, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1138-25UG |
| Description | Anti-HPCAL1 Antibody, clone 5A5 |
| Alternate Names |
|
| Background Information | Hippocalcin-like protein 1 (UniProt: P37235; also known as Calcium-binding protein BDR-1, HLP2, Visinin-like protein 3, VILIP-3) is encoded by the HPCAL1 (also known a BDR1) gene (Gene ID: 3241) in human. HPCAL1 is a lipid-anchor membrane protein of the visinin-like subfamily of neural calcium sensor and is involved in the calcium-dependent regulation of rhodopsin phosphorylation. It is structurally related to Ca2+-myristoyl switch proteins that contain four EF-hand motifs and a covalently attached N-terminal myristoyl group. HPCAL1 is also expressed in Purkinje cells of the cerebellum and hippocampus where it is believed to regulate synaptic plasticity relevant for learning and memory. Clone 5A5 can detect HPCAL1 in formalin-fixed 293 cells and in pancreatic cell lines. (Ref.: Chen, Y., et Al. (2014). Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 33(1); 20-27; Li, C., et Al. (2016). PLoS One 11(11); e0165921). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without azide. |
| Applications | |
|---|---|
| Application | Detects Hippocalcin-like protein 1 using this mouse monoclonal Anti-HPCAL1, clone 5A5, Cat. No. MABC1138, tested for use in Western Blotting, Immunohistochemistry (Paraffin), ELISA and Immunocytochemistry. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected HPCAL1 in Immunocytochemistry applications (Chen, Y., et. Al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7). ELISA Analysis: A representative lot detected HPCAL1 in ELISA applications (Chen, Y., et. Al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7). Immunohistochemistry (Paraffin) Analysis: A 1:50-250 Dilution from a representative lot detected HPCAL1 in human kidney and human cerebellum tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | Native and denatured human HPCAL1. |
| Clone | 5A5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 5A5 specifically detects Hippocalcin-like protein 1 (HPCAL1) in human cells. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein g purified |
| UniProt Number |
|
| Molecular Weight | ~22 kDa observed; 22.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Panc-1 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected HPCAL1 in Panc-1 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |