Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1191-100UG |
| Description | Anti-Sodium-Iodide Symporter Antibody, clone ARN1 |
| Alternate Names |
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| Background Information | Sodium/iodide cotransporter (UniProt: Q92911; also known as Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter, Solute carrier family 5 member 5) is encoded by the SLC5A5 (also known as NIS) gene (Gene ID: 6528) in human. Sodium/iodide cotransporter is a multi-pass membrane protein that mediates iodide uptake in thyroid gland. It is expressed primarily in thyroid tissue, but is present in lower levels in mammary gland and ovary. It is found in the basolateral membrane of thyroid follicular cells where it mediates efficient iodide uptake from the bloodstream into the thyroid using the sodium gradient generated by Na+/K+-ATPase. It transports one iodide ion along with two sodium ions following the inwardly directed sodium gradient. Sodium/iodide cotransporter expression is significantly reduced in tumors. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. NIS gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Mutations in SLC5A5 gene are known to cause thyroid dyshormonogenesis (TDH1) that is characterized by the inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland, leading to congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 140 mm Tris and 180 mm Glycine (pH 7.4) without azide. |
| Applications | |
|---|---|
| Application | Anti-Sodium-Iodide Symporter, clone ARN1, Cat. No. MABC1191, is a mouse monoclonal antibody that detects Sodium/iodide cotransporter and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Sodium-Iodide Symporter in human stomach tissue sections. Immunohistochemistry (Paraffin) Analysis: A representative lot detected Sodium-Iodide Symporter in Immunohistochemistry applications (Tazebay, U.H., et. Al. (2000). 6(8):871-8). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Sodium/iodide cotransporter. |
| Epitope | C-terminus |
| Clone | ARN1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone ARN1 is a mouse monoclonal antibody that detects Sodium/iodide cotransporter in human cells. It targets an epitope within 13 amino acids from the C-terminal region. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~68 kDa observed; 68.67 kda calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in MDCK cells transduced with human WT NIS. Western Blotting Analysis: 1 µg/mL of this antibody detected Sodium-Iodide Symporter in MDCK cells transduced with human WT Sodium-Iodide Symporter (NIS). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |