The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Anti-Rae1, Clone 14g7.1, Cat. No. Mabc1233, Is A Mouse Monoclonal Antibody That Detects Mrna Export Factor (Rae1) And Has Been Tested For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
mRNA export factor (UniProt: P78406; also known as Rae1 protein homolog, mRNA-associated protein mrnp 41) is encoded by the RAE1 (also known as MRNP41) gene (Gene ID: 8480) in human. mRNA export factor (UniProt: P78406; also known as RAE1 protein homolog, mRNA-associated protein mrnp 41) is encoded by the RAE1 (also known as MRNP41) gene (Gene ID: 8480) in human. Rae1 is a member of the superfamily of WD-repeat proteins that plays a role in mitotic bipolar spindle formation. It contains seven WD repeats. Rae1 is also reported to bind to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains, including WD-repeats. Also, the COOH-Terminal non- WD-repeat extension of Rae1 Is shown to be essential for NUP98 binding. Rae1 plays a role in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. It is recruited from interphase nuclei to spindle microtubules during mitosis. RAE1 also interacts with NUMA1 via N-terminal end of the coiled-coil domain and this interaction promotes spindle formation in mitosis. (Ref.: Pritchard, CEJ et al. (1999). J. cell Biol. 145(2); 237-254).
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-Rae1, clone 14G7.1, Cat. No. MABC1233, is a mouse monoclonal antibody that detects mRNA export factor (Rae1) and has been tested for use in Western Blotting.
Key Applications
Western Blotting
Biological Information
Immunogen
GST/His-tagged recombinant fragment corresponding to 179 amino acids from the C-terminal half of human mRNA export factor (Rae1).
Clone
14G7.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 14G7.1 specifically detects human mRNA export factor (Rae1). it targets an epitope with in the C-terminal half.
Isotype
IgG2bκ
Species Reactivity
Human
Species Reactivity Note
Human.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001015885
Gene Symbol
RAE1
MRNP41
Purification Method
Protein G purified
UniProt Number
P78406
Molecular Weight
~40 kDa observed; 40.97 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rae1 in HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
