The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Anti-Trail/Cd253 Antibody, Clone 6d12.2 Is An Antibody Against Trail/Cd253 For Use In Flow Cytometry & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
Tumor necrosis factor ligand superfamily member 10
Apo-2 ligand
Apo-2L
TNF-related apoptosis-inducing ligand
Protein TRAIL
CD253
Background Information
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel member in the TNF family was recently identified and designated TRAIL (for TNF-related apoptosis-inducing ligand) and Apo-2L (for Apo-2 ligand). TRAIL is a type II membrane protein and is expressed in a variety of human tissues. Two novel death domain containing receptors DR4 and DR5 have been identified as the receptor for TRAIL. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-TRAIL/CD253 Antibody, clone 6D12.2 is an antibody against TRAIL/CD253 for use in flow cytometry & IHC.
Key Applications
Flow Cytometry
Immunohistochemistry
Application Notes
Flow Cytometry Analysis: 4 µg of this antibody detected TRAIL/CD253 in 1X10E6 human PBMC, which were treated with a proinflammatory stimulus.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TRAIL/CD253 in human prostate cancer sections. (Note: This antibody detects TRAIL/CD253 in cancer tissues and not in normal tissues.).
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human TRAIL/CD253.
Clone
6D12.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001177871
Gene Symbol
TNFSF10
APO2L
TRAIL
Purification Method
Protein G Purified
UniProt Number
P50591
Molecular Weight
33 kDa calculated
Product Usage Statements
Quality Assurance
Evaluated by Flow Cytometry in HeLa cells, which were treated with a proinflammatory stimulus.
Flow Cytometry Analysis: 4 µg of this antibody detected TRAIL/CD253 in 1X10E6 HeLa cells, which were treated with a proinflammatory stimulus.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
