| Description | |
|---|---|
| Catalogue Number | MABC1596-25UG |
| Description | Anti-Tex19.1 Antibody, clone 7TEX-1F11 |
| Alternate Names |
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| Background Information | Testis-expressed protein 19.1 (UniProt: Q99MV2; also known as mTex19.1, Testis-expressed protein 19A) is encoded by the Tex19.1 (also known as Tex19, Tex19a) gene (Gene ID: 73679) in murine species. Tex19.1 resides in the germline to repress transposable genetic elements and maintain genomic stability. It is expressed in testis, placenta, ovary, and in pluripotent stem cells. In testis its expression is highest in mitotic spermatogonia and decreases as spermatocytes progress through meiosis. Tex19.1 expression is reported to be down-regulated by Deleted in azoospermia-like (DAZL) protein, which binds to 3UTR of Tex19.1 mRNAs and repress its translation. Tex19.1 is required during spermatogenesis and placenta development where it participates in the repression of retrotransposable elements and prevents their mobilization. Tex19.1, along with its paralog, Tex19.2, is shown to collaborate with the Piwi-interacting RNA (piRNA) pathway, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins. Embryos with mutations in Tex19.1 gene exhibit intra-uterine growth retardation and have small placentas due to a reduction in the number of spongiotrophoblast, glycogen trophoblast and sinusoidal trophoblast giant cells. Loss of Tex19.1 is shown to cause growth defect and early post-natal lethality. Male animals with deficiency of both Tex19.1 and Tex19.2 display impaired spermatogenesis, small testes, and are infertility. |
| Product Information | |
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| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-Tex19.1, clone 7TEX-1F11, Cat. No. MABC1596, is a mouse monoclonal antibody that detects Testis-expressed protein 19.1 and has been tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
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| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Tex19.1 in embryonic mouse brain and embryonic mouse gut tissue sections. Immunocytochemistry Analysis: A representative lot detected Tex19.1 in Immunocytochemistry applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14). ELISA Analysis: A representative lot detected Tex19.1 in ELISA applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14). Immunoprecipitation Analysis: A representative lot immunoprecipitated Tex19.1 in Immunoprecipitation applications (Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474). Western Blotting Analysis: A representative lot detected Tex19.1 in Western Blotting applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14;Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full-length recombinant mouse Testis-expressed protein 19.1 (Tex 19). |
| Clone | 7TEX-1F11 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 7TEX-1F11 detects murine Testis-expressed protein 19.1 (Tex 19). |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 40.40 kDa calculated. |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in mouse testis tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Tex19.1 in mouse testis tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |