The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Anti-Pdl-1/Cd274 Antibody, Clone 3f2.1 Is An Antibody Against Pdl-1/Cd274 For Use In Western Blotting & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
Programmed cell death 1 ligand 1 (PDL-1) is a B7 related protein belonging to the BTN/MOG family. PDL-1 is essential for T-cell proliferation and production of IL10 and IFNG. PDL-1 reduces the secretion of IL-2 and IL-10 from memory T cells by inhibiting cell-mediated immune responses. PDL-1 may be involved in reducing allogenic CD4+ memory T-cell responses to endothelial cells. PDL-1 is highly expressed in heart, skeletal muscle, placenta, and lung and weakly expressed in thymus, spleen, kidney, and liver.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-PDL-1/CD274 Antibody, clone 3F2.1 is an antibody against PDL-1/CD274 for use in western blotting & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50 and 1:500 dilution from a representative lot detected PDL-1/CD274 in human tonsil and skeletal muscle tissue, respectively.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the extracellular domain of human PDL-1/CD274.
Epitope
extracellular domain
Clone
3F2.1
Concentration
1 mg/mL
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001254635
Gene Symbol
CD274
B7H1
PDCD1L1
PDCD1LG1
PDL1
Purification Method
Protein G Purified
UniProt Number
Q9NZQ7
Molecular Weight
~40 kDa observed. The calculated molecular weight is 33 kDa; however, this protein may be observed at higher molecular weights to due to heavy glycosylation.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human skeletal muscle tissue.
Western Blotting Analysis: 1 µg/mL of this antibody detected PDL-1/CD274 in 10 µg of human skeletal muscle tissue.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
