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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC521 |
| Description | Anti-XBP1s Antibody, clone 143F (active form) |
| Alternate Names |
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| Background Information | XBP-1, also known as X-box-binding protein 1 or Tax-responsive element-binding protein 5, and encoded by the gene XBP1/TREB5/XBP2, is a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP-1 also acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). XBP-1 binds DNA preferentially at CRE-like elements but also to some TPA response elements (TRE). XBP-1 is expressed in the nucleus. Defects in XBP-1 may be associated with Major affective disorder 7 (MAFD7) a major psychiatric disorder that is characterized by severe mood swings, with fluctuation between two abnormal mood states (manic or major depressive episode). Mania is accompanied by symptoms of euphoria, irritability, or excitation, whereas depression is associated with low mood and decreased motivation and energy. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-XBP1s Antibody, clone 143F (active form) is an antibody against XBP1s for use in IHC & western blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analyis: A representative lot from an independent laboratory detected XBP-1S, clone 143F (active form) in SK-mm-2, KARPAS 620, NCI-H929, and LP-1 cell lysates (Maestre, L., et Al. (2009). Haematologica. 94(3):419-422.). Immunohistochemistry Analysis: A representative lot from an independent laboratory detected XBP-1S, clone 143F (active form) in human tonsil and lymphoplasmacytic cells (Maestre, L., et Al. (2009). Haematologica. 94(3):419-422.). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the C-terminus of human XBP-1S, clone 143F (active form). |
| Epitope | C-terminus |
| Clone | 143F |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | 29 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human skin tissues. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected XBP-1S, clone 143F (active form) in human skin tissues. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |