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Millipore This Anti-Trp1/Tyrp1 Antibody, Clone Ta99, Azide Free Is Validated For Use In Western Blotting, Ihc, Blocking Of Inteferring Antibodies, Ip & Flow Cytometry For The Detection Of Trp1/Tyrp1. - Mill (Additional S&H or Hazmat Fees May Apply)
WB, IHC, Blocking of Intefering Antibodies, IP, FC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
MABC592
Description
Anti-TRP1/TYRP1 Antibody, clone TA99, Azide Free
Alternate Names
5,6-dihydroxyindole-2-carboxylic acid oxidase
DHICA oxidase
Catalase B
Glycoprotein 75
Melanoma antigen gp75
Tyrosinase-related protein 1
TRP
TRP-1
TRP1
Background Information
TRP1/TYRP1, also known as 5,6-dihydroxyindole-2-carboxylic acid oxidase, DHICA, Catalase B, Glycoprotein 75, Melanoma antigen gp75, or Tyrosinase-related protein 1 (TRP/TRP-1/TRP1), and encoded by the gene TYRP1/CAS2/TYRP/TYRRP, is involved in melanin synthesis and is the enzyme that oxidizes DHICA into indole-5,6-quinone-2-carboxylic acid in the pathway. TRP1/TYRP1 is critical for skin/eye/hair pigmentation. Mutations are associated with Albinism. TRP1/TYRP1 is regulated by the microphthalmia-associated transcription factor (MITF). MITF is a master regulator of pigmentation and a target of β-catenin in Wnt/β-catenin signaling during melanocyte differentiation. TRP1/TYRP1 is also regulated by the microRNA miR-145, and this microRNA is being examined as a possible therapy for hyperpigmentation. TRP1/TYRP1 is expressed as a membrane protein in melanosomes and endosomes in pigment cells.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.
Quality Level
MQ100
Applications
Application
This Anti-TRP1/TYRP1 Antibody, clone TA99, Azide Free is validated for use in western blotting, IHC, blocking of inteferring antibodies, IP & flow cytometry for the detection of TRP1/TYRP1.
Key Applications
Western Blotting
Immunohistochemistry
Blocking of Intefering Antibodies
Immunoprecipitation
Flow Cytometry
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TRP1/TYRP1 in human melanoma tissue.
Blocking of Interferring Antibodies Analysis: A representative lot from an independent laboratory suppressed the growth of subcutaneous B16 tumors (Patel, D., et al. (2008). Anticancer Res. 28(5A):2679-2686.).
Activity Assay Analysis: A representative lot from an independent laboratory improves anti-tumor efficacy by augmenting systemic CD8+T cell responses to tumor cells (Saenger, Y. M., et al. (2008). Cancer Res. 68(23):9884-9891.).
Immunoprecipiptation Analysis: A representative lot from an independent laboratory immunoprecipiated TRP1/TYRP1 from B16 cell lysate (Srinivasan, R., et al. (2002). Cancer Immun. 19(2):8.).
Biological Information
Immunogen
Whole cells corresponding to human TRP1/TYRP1.
Clone
TA99
Concentration
1 mg/mL
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Mouse
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000541
Gene Symbol
TYRP1
CAS2
TYRP
TYRRP
Purification Method
Protein G Purified
UniProt Number
P17643
Molecular Weight
~60 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blottng in mouse skin tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TRP1/TYRP1 in 10 µg of mouse skin tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
