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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Mk | WB, IHC, IF, FC, ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC697 |
| Description | Anti-ACLY, clone 5F8D11 Antibody |
| Alternate Names |
|
| Background Information | ATP citrate synthase, also known as ATP-citrate (pro-S-)-lyase (ACL) or Citrate cleavage enzyme, and encoded by the gene name ACLY is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal in buffer containing PBS with up to 0.1% sodium azide and 0.5% protein stabilizer. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-ACLY, clone 5F8D11 Antibody is validated for use in western blotting, IHC, immunofluorescence, flow cytometry & ELISA for the detection of ACLY. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ACLY in esophageal cancer tissue. Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected ACLY in HeLa cells. Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected ACLY in HeLa cells. ELISA: A 1:10,000 dilution from a representative lot was used on control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct). |
| Biological Information | |
|---|---|
| Immunogen | Purified recombinant fragment of human ACLY expressed in E. Coli |
| Clone | 5F8D11 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| Molecular Weight | ~125 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates. Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ACLY in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |