Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IHC, Inhibition, IH(P) | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC961-25UL |
| Description | Anti-RTN4IP1 Antibody, clone NIMP‐R14 |
| Alternate Names |
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| Background Information | Reticulon-4-interacting protein 1, mitochondrial (UniProt: Q924D0; also known as NOGO-interacting mitochondrial protein, RTN4IP1) is encoded by the Rtn4ip1 (also known as Nimp) gene in murine species. RTN4IP1 is an outer mitochondrial membrane protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. This interaction may be important for reticulon-induced inhibition of neurite growth. RNT4IP1 plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. It is widely expressed in mitochondria-enriched tissues and co-localizes with the endoplasmic reticulum HSPA5 at spots corresponding to contacts with mitochondria. Mutations in Rtn4ip1 gene can cause optic atrophy 10 that leads to vision loss sometime in early childhood. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2b in PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Reticulon-4-interacting protein 1, mitochondrial using this rat monoclonal Anti-RTN4IP1, clone NIMP‐R14 , Cat. No. MABC961-25UL, validated for use in Immunohistochemistry, Inhibition and Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected RTN4IP1 in Flow Cytometry applications (Lopez, A.F., et. Al. (1984). Br J Haematol. 57(3):489-94; Taylor, P.R., et. Al. (2014). Nat Immunol. 15(2):143-51). Inhibition Analysis: A representative lot detected RTN4IP1 in Inhibition applications (Tacchini-Cottier, F., et. Al. (2000). J Immunol. 165(5):2628-36; de Vries, B., et. Al. (2003). J Immunol. 170(7):3883-9; Chang, H.R., et. Al. (1993). J Leukoc Biol. 53(6):636-9; Mentzel, S., et. Al. (1999). Kidney Int. 55(4):1335-47). Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected RTN4IP1 in mouse lung tissue. Immunohistochemistry Analysis: A representative lot detected RTN4IP1 in Immunohistochemistry applications (Lopez, A.F., et. Al. (1984). Br J Haematol. 57(3):489-94). Immunohistochemistry Analysis: A representative lot detected RTN4IP1 in Immunohistochemistry applications (Lopez, A.F., et. Al. (1984). Br J Haematol. 57(3):489-94.; de Vries, B., et. Al. (2003). J Immunol. 170(7):3883-9; Chang, H.R., et. Al. (1993). J Leukoc Biol. 53(6):636-9). Immunocytochemistry Analysis: A representative lot detected RTN4IP1 in Immunocytochemistry applications (Lopez, A.F., et. Al. (1984). Br J Haematol. 57(3):489-94.; Taylor, P.R., et. Al. (2014). Nat Immunol. 15(2):143-51). |
| Biological Information | |
|---|---|
| Immunogen | Purified BALB/c mouse neutrophils. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone NIMP‐R14 is a rat monoclonal antibody that detects Reticulon-4-interacting protein 1 in murine species. |
| Isotype | IgG2b |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in mouse spleen tissue. Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected RTN4IP1 in mouse spleen tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |