Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1013-25UG |
| Description | Anti-RRP8 Antibody, clone 5C7.1 |
| Alternate Names |
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| Background Information | Ribosomal RNA-processing protein 8 (UniProt: O43159; also known as Cerebral protein 1, Nucleomethylin) is encoded by the RRP8 (also known as KIAA0409, NML, hucep-1) gene (Gene ID: 23378) in human. Ribosome biosynthesis is one of the most energy-consuming cellular process, which adapts to changes in intracellular energy status. Ribosomal RNA-processing protein 8 is a novel nuclear protein that serves as an essential component of the energy-dependent nucleolar silencing) complex (eNoSC) that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation or upon elevation of NAD+/NADP+ ratio and activates SIRT1 that leads to deacetylation of histone H3 followed by dimethylation of H3 at Lysine 9 (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-RRP8, clone 5C7.1, Cat. No. MABE1013, is a highly specific mouse monoclonal antibody, that targets Ribosomal RNA-processing protein 8 and has been tested for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RRP8 in HeLa, A431, HUVEC, and NIH/3T3 cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to 98 amino acids from the C-terminal half of human Ribosomal RNA-processing protein 8. |
| Clone | 5C7.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 5C7.1 specifically detects Ribosomal RNA-processing protein 8. It targets an epitope with in the C-terminal half. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~53 kDa observed; 50.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected RRP8 in HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |