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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IH(P) | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1045 |
| Description | Anti-CTIP2/BCL11B Antibody, clone 25B6 |
| Alternate Names |
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| Background Information | B-cell lymphoma/leukemia 11B (UniProt Q99PV8; also known as B-cell CLL/lymphoma 11B, BCL-11B, COUP-TF-interacting protein 2, mRit1, Radiation-induced tumor suppressor gene 1 protein) is encoded by the Bcl11b (also known as Ctip2, Rit1) gene (Gene ID 58208) in murine species. CTIP2/CBL11B is a C2H2 zinc finger protein that binds GC-rich response elements and is reported to associate with a variety of cofactors, including the corepressor complexes NuRD, Sirt1, histone acetyltransferase (HAT) p300, functioning both as a transcriptional repressor and activator. CTIP2/CBL11B is required at multiple developmental checkpoints for T cell commitment and maintenance. CTIP2/CBL11B expression is initiated at double-negative 2 (DN2) stage of T cell development and is maintained in mature T lymphocytes, with lower levels found in NK cells, but not in B cells, myeloid or dendritic cells. Bcl11b-/- mice die shortly after birth due to a blockade of thymocyte development at the DN2 stage. Bcl11b-deficient DN2 thymocytes are highly proliferative and retain the potential to differentiate into NK cells and myeloid lineages, but not mature T-cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in buffer containing 150 mM NaCl, 15 mM HEPES, pH 7.5, with 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CTIP2/BCL11B Antibody, clone 25B6, Cat. No. MABE1045, is a highly specific rat monoclonal antibody that targets CTIP2 and has been tested in Immunofluorescence, Immunohistochemistry, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:240 dilution from a representative lot detected CTIP2/BCL11B in mouse brain tissue sections. Immunofluorescence Analysis: A representative lot detected CTIP2 localization in 4% paraformaldehyde-fixed floating embryonic (E14 & E16) and postnatal (P3 & P6) mouse brain sections (Arlotta, T., et al. (2005). Neuron. 45(2):207-221). Western Blotting Analysis: A representative lot detected doublet CTIP2 bands, representing two spliced isoforms, in Jurkat nuclear extracts as well as in SIRT1 immunoprecipitate (Senawong, T., et al. (2003). J. Biol. Chem. 278(44):43041-43050). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant full-length mouse CTIP2 Beta isoform (Senawong, T., et al. (2003). J. Biol. Chem. 278(44):43041-43050). |
| Clone | 25B6 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 25B6 recognizes both the Alpha and Beta spliced isoforms of human and mouse CTIP2/BCL11B. |
| Isotype | IgG2a |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~96 kDa observed. 95.52/94.57 kDa (human/mouse isoform Alpha) and 88.48/87.34 kDa (human/mouse isoform Beta) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected CTIP2/BCL11B in 10 µg of HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |