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Millipore Anti-Mono-ADP-Ribose Binding Reagent, Cat. No. Mabe1076, Is A Reagent That Targets Mono (ADP-Ribose) Modified Proteins And Has Been Tested In DOT Blot And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Anti-mono-ADP-ribose binding reagent is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). It is useful for the affinity detection of both mono-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and DOT blot analysis. The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.
Product Information
Format
Purified
Presentation
Purified from E. coli and supplied in buffer containing 10 mm Tris pH 7.5, 0.2 M NaCl, 10 mm Imidazole, 1 mm PMSF, 1 mm beta-Mercaptoethanol, with 10% glycerol without preservatives.
Quality Level
MQ100
Applications
Application
Anti-mono-ADP-ribose binding reagent, Cat. No. MABE1076, is a reagent that targets mono (ADP-ribose) modified proteins and has been tested in DOT Blot and Western Blotting.
Key Applications
DOT Blot
Western Blotting
Application Notes
Western Blotting Analysis: 0.4 µg/mL of a representative lot detected mono-ADP-ribosylated proteins.
DOT Blot Analysis: A representative lot detected mono-,-ADP-ribose modified proteins.
DOT Blot Analysis: A representative lot detected mono-ADP ribosylated proteins. (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
Western Blotting Analysis: A representative lot detected mono-ADP-ribose modified protein by Wester blotting (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
Biological Information
Concentration
Please refer to lot specific datasheet.
Host
E. coli
Specificity
This reagent binds to mono-ADP ribosylated proteins.
Species Reactivity
Mouse
Human
Species Reactivity Note
Mouse, Human.
Antibody Type
Monoclonal Antibody
Modifications
ADP-ribosylation
Purification Method
Immobilized Metal Affinity Chromatography (IMAC)
UniProt Number
N/A
Molecular Weight
Variable depending on the target proteins and the extent of ADP-ribosylation.
Product Usage Statements
Quality Assurance
Evaluated by Gel Electrophoresis.
Gel Electrophoresis Analysis: 0.5 µg of this binding reagent was analyzed on GEL Electrophoresis to test for purity.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
