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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Rhesus Macaque | ELISA, FC, ICC, IHC, IP | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1109 |
| Description | Anti-HHV-8 LNA-1 Antibody, clone LN53 |
| Alternate Names |
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| Background Information | Human herpesvirus-8//Kaposis sarcoma-associated herpesvirus (HHV-8/KSHV) and macaque retroperitoneal fibromatosis herpesvirus (RFHV) were originally identified in in AIDS patients in association with Kaposis sarcoma (KS) and in retroperitoneal fibromatosis (RF) tumor lesions of macaques with simian AIDS, respectively. The most prominent protein expressed in cells latently infected with KSHV and RFHV is the orf73 gene product known as the latency-associated nuclear antigen (LANA or LNA). LANA is a nuclear protein that functions to ensure the maintenance of the viral genome by tethering the viral episomal DNA to host cell chromosomes. LANA also regulates the cellular transcription program in host cells through interaction with a number of cellular proteins, including transcriptional regulators and known tumor suppressors, p53 and pRB. In addtion, LANA directly influences the viral transcription program and helps maintain the latent state of the virus by inhibiting viral replication. KSHV LANA consists of a serine- and proline-rich N-terminal domain with a nuclear localization signal (NLS), a chromatin-binding motif (CBM), and domains responsible for interaction with the transcription regulators, mSin3 complex and Sp1. The central domain contains several highly repetitive acidic regions that vary in length and are responsible for the size variation of LANA proteins from different KSHV isolates that can range from 1003 to 1162 amino acids. The proline-rich C-terminal third domain contains another NLS and is responsible for LANA dimerization and binding to the terminal repeats (TR) of the viral genomic DNA. The C-terminal domain is responsible for interaction with tumor suppressors pRB and p53. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mm NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-HHV-8 LNA-1, clone LN53, Cat. No. MABE1109, is a highly specific rat monoclonal antibody that targets human KSHV/HHV8 and simian RFHV Orf73 latency-associated nuclear antigen (LANA or LNA) and has been tested in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitati |
| Key Applications |
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| Application Notes | ELISA Analysis: A representative lot detected HHV-8 LNA-1 recombinant constructs containing the repetitive region a.a. 803-929 (Kellam, P., et Al. (1999). J. Virol. 73(6): 5149 5155). Flow Cytometry Analysis: A representative lot detected HHV-8 LNA-1 immunoreactivity in BCP-1, BC-3, and HBL-6 cells, but not in Daudi or Ramos cells (Kellam, P., et Al. (1999). J. Virol. 73(6): 5149 5155). Immunocytochemistry Analysis: A representative lot detected the transiently expressed KHSV ORF73 (HHV-8 LANA or LNA-1) as well as rhesus macaque retroperitoneal fibromatosis herpesvirus ORF73 (RFHV LANA) in the nuclei of transfected COS cells by fluorescent immunocytochemistry. No reactivity was detected toward pig-tailed macaque MneRV2 or rhesus macaque RRV LANA proteins (Burnside, K.L., et Al. (2006). Virology. 354(1):103-115). Immunocytochemistry Analysis: A representative lot detected HHV-8 LNA-1-positive nuclear bodies in primary effusion lymphoma (PEL) cell line BCP-1 by fluorescent immunocytochemistry, but not in Daudi or Ramos cells (Kellam, P., et Al. (1999). J. Virol. 73(6): 5149 5155). Immunohistochemistry Analysis: A representative lot detected RFHVMn LANA immunoreactivity in the nuclei of retroperitoneal fibromatosis (RF) tumor cells by immunohistochemistry staining of formalin-fixed, paraffin-embedded RF biopsies from an SIV-infected pig-tailed macaque. No immunoreactivity was detected using normal macaque jejunum tissue sections (Burnside, K.L., et Al. (2006). Virology. 354(1):103-115). Immunohistochemistry Analysis: Representative lots immunostained cells latently infected by HHV-8 by immunohistochemistry staining of paraffin-embedded patch, plaque, and nodular Kaposi s sarcoma (KS), multicentric Castleman s disease (MCD), and primary effusion lymphoma (PEL) tissue sections (Codish, S., et Al. (2000). Am. J. Hematol. 65(4):310-314; Dupin, N., et Al. (2000). Blood. 95(4):1406-1412; Dupin, N., et Al. (1999). Proc. Natl. Acad. Sci. U. S. A. 96(8):4546-4551). Immunohistochemistry Analysis: A representative lot detected nuclear HHV-8 LNA-1 immunoreactivity in cells within the nodular Kaposi s sarcoma (KS) lesion, but not in the surrounding dermis by immunohistochemistry staining of paraffin-embedded classical KS tumor sections (Kellam, P., et Al. (1999). J. Virol. 73(6): 5149 5155). Immunoprecipitation Analysis: A representative lot immunoprecipitated 220/230 kDa HHV-8 LNA-1 from primary effusion lymphoma (PEL) cell line BC-3, but not from Ramos cells (Kellam, P., et Al. (1999). J. Virol. 73(6): 5149 5155). Western Blotting Analysis: A representative lot detected the transiently expressed KHSV ORF73 (HHV-8 LANA or LNA-1) as well as rhesus macaque retroperitoneal fibromatosis herpesvirus ORF73 (RFHV LANA) in lysate from transfected COS cells. No reactivity was detected toward pig-tailed macaque MneRV2 or rhesus macaque RRV LANA proteins (Burnside, K.L., et Al. (2006). Virology. 354(1):103-115). Western Blotting Analysis: A representative lot detected the 220/230 kDa HHV-8 LNA-1 doublet in lysate from primary effusion lymphoma (PEL) cell line BC-3, but not from Ramos cells (Kellam, P., et Al. (1999). J. Virol. 73(6): 5149 5155). |
| Biological Information | |
|---|---|
| Immunogen | Affinity-purified GST fusions of HHV-8 LNA-1 C-terminal fragments (Kellam, P., et Al. (1999). J. Virol. 73(6): 5149 5155). |
| Clone | LN53 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone LN53 recognizes repetitive glutamic acid structural motifs EQEQE and EPEPE found in the Glu-rich repeat region of human Kaposis sarcoma-associated herpesvirus (KSHV or HHV-8) and simian retroperitoneal fibromatosis herpesvirus (RFHV) Orf73 latency-associated nuclear antigen (LANA or LNA), respectively. |
| Isotype | IgG2cκ |
| Species Reactivity |
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| Species Reactivity Note | Human KSHV (HHV8) and simian RFHV. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified. |
| UniProt Number |
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| Molecular Weight | 116/135 kDa (KSHV LNA) and 118 kDa (RFHV LNA) prediced, ~220/230 kDa doublet (KSHV) and ~240 kDa doublet (RFHV) reported (Burnside, K.L., et Al. (2006). Virology. 354(1):103-115; Kellam, P., et Al. (1999). J. Virol. 73(6): 5149 5155). The larger-than-predicted apparent molecular weight is most likely caused by the internal acidic repeat region sequence (Rainbow, L., et Al. (1997). J. Virol. 71(8):5915-5921). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in BCBL-1 cells. Immunocytochemistry Analysis (ICC): 1 µg/mL of this antibody immunostained HHV-8 LNA-1-positive nuclear bodies in Kaposis sarcoma-associated herpesvirus/human herpesvirus 8- (KSHV/HHV8-) infected BCBL-1 lymphoma cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |