Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Virus | ICC, IF | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1134-25UL |
| Description | Anti-dsRNA Antibody, clone rJ2 |
| Alternate Names |
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| Background Information | Double-stranded RNA (dsRNA) is a viral product that induces innate immunity, leading to the production of interferon (IFN) alpha and beta, which can lead to the activation of hundreds of IFN-stimulated genes that confer resistance to viruses. dsRNA of more than 30-bp length are reported to be is a key activator of the innate immune response against viral infections. dsRNA is produced by positive-strand RNA viruses, dsRNA viruses, and DNA viruses. However, negative-strand RNA viruses are not shown to generate any significant dsRNA signals. The interaction of the host cell with dsRNA can occur in several ways. Mainly, specific receptors activate the synthesis of IFN alpha and beta, antiviral proteins, and dsRNA-activated enzymes that can block viral replication. dsRNA replication is shown to occur in the cytoplasm for all dsRNA viruses. This dsRNA-specific mouse monoclonal antibody specifically recognizes dsRNA of more than 40-bp length. Clone rJ2 has been used to detect dsRNA intermediates of multiple types of viruses, including Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, and Polio virus in biological samples. It can be useful in understanding how anti-viral responses are initiated and what how viruses overcome and avoid these antiviral therapies. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Presentation | Mouse monoclonal antibody in supernatant without preservatives. |
| Applications | |
|---|---|
| Application | Anti-dsRNA, clone rJ2, Cat. No. MABE1134, is a mouse monoclonal antibody that detects double stranded RNA (dsRNA) and has been tested for use in Immunocytochemistry and Immunofluorescence. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A representative lot detected dsRNA in Immunofluorescent applications (Savidis, g., et. Al. (2016). Cell Rep. 15(11):2323-30; Savidis, g., et. Al. (2016). Cell Rep. 16(1):232-246). |
| Biological Information | |
|---|---|
| Immunogen | Double stranded RNA produced by positive sense genome viruses. |
| Clone | rJ2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone rJ2 specifically recognizes double stranded RNA (dsRNA) of greater than 40 bp in length that is generated during the replication of positive sense genome viruses. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Virus. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Unpurified |
| Molecular Weight | N/A |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in Dengue virus infected A549 cells. Immunocytochemistry Analysis: A 1:60 dilution of this antibody detected dsRNA in Dengue virus infected A549 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |