Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1151 |
| Description | Anti-OASIS (CREB3L1) Antibody, clone 10H1 |
| Alternate Names |
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| Background Information | Cyclic AMP-responsive element-binding protein 3-like protein 1 (UniProt: Q96BA8; also known as cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, OASIS) is encoded by the CREB3L1 (also known as OASIS, PSEC0238) gene (Gene ID: 90993) in human. OASIS is a single-pass type II membrane protein that serves as a transcription factor and is involved in unfolded protein response (UPR). It binds the DNA consensus sequence 5-GTGXGCXGC-3. It has a cytoplasmic domain (aa 1-374), a helical domain (aa 375-395), and a luminal domain (aa 396-519). In the absence of endoplasmic reticulum (ER) stress, it is inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. However, in response to ER stress, it is transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. OASIS also plays a critical role in bone formation through the transcription of COL1A1, and possibly COL1A2, and the secretion of bone matrix proteins. It directly binds to the UPR element (UPRE)-like sequence in an osteoblast-specific COL1A1 promoter region and induces its transcription. OASIS is also required to protect astrocytes from ER stress-induced cell death. In astrocytes, it binds to the cAMP response element (CRE) of the BiP/HSPA5 promoter and participate in its transcriptional activation. Mutations in CREB3L1 gene are reported to cause osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-OASIS (CREB3L1), clone 10H1, Cat. No. MABE1151, is a highly specific mouse monoclonal antibody that targets Cyclic AMP-responsive element-binding protein 3-like protein 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected OASIS (CREB3L1) in human pancreas and human tonsil tissues. Immunohistochemistry Analysis: A representative lot detected OASIS (CREB3L1) in Immunohistochemistry applications (Denard, B., et. Al. (2015). PLoS One. 10(6):e0129233). Western Blotting Analysis: A representative lot detected OASIS (CREB3L1) in Western Blotting applications (Denard, B., et. Al. (2015). PLoS One. 10(6):e0129233; Chen, Q., et. Al. (2016). Mol Cell. 63(4):567-78). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear Peptide corresponding to 35 amino acids from the N-terminal region of human OASIS (CREB3L1). |
| Clone | 10H1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 10H1 detects Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) in human tissues. it targets an epitope within 35 amino acids from the N-terminal region. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Rat, Mouse, Bovine based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 57.00 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human prostate cancer tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected OASIS (CREB3L1) in human prostate cancer tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |