Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | AHm | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1153-25UG |
| Description | Anti-MRE11 Antibody, clone 15B8.1E7.6 |
| Alternate Names |
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| Background Information | Double-strand break repair protein MRE11A (UniProt: Q61216; also known as MmMRE11A, Meiotic recombination 11 homolog 1, MRE11 homolog 1, Meiotic recombination 11 homolog A, MRE11 homolog A) is encoded by the Mrer11a (also known as Mre11) gene (Gene ID: 17535) in murine species. MRE11A a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. It localizes to discrete nuclear foci after treatment with genotoxic agents. MRE11A is a component of the MRN complex that possesses single-strand endonuclease activity and double-strand-specific 3-5 exonuclease activity. MRE11A forms a complex with the RAD50 homolog, which is required for non-homologous joining of DNA end. RAD50 may be required to bind DNA ends and hold them in close proximity, which facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. In conjunction with a DNA ligase, MRE11A is reported to promote the joining of non-complementary ends in vitro using short homologies near the ends of the DNA fragments. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified armenian hamster monoclonal antibodyIgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MRE11, clone 15B8.1E7.6 , Cat. No. MABE1153-25UG, is a highly specific armenian hamster monoclonal antibody, that targets Double-strand break repair protein MRE11A and has been tested in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1 µg/mL from a representative lot detected MRE11 in 10 µg of mouse embryo fibroblast cell lysate (MEFs). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant fragment corresponding to 540 amino acids from murine Double-strand break repair protein MRE11A. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Armenian Hamster |
| Specificity | Clone 15B8.1E7.6 detects Double-strand break repair protein MRE11A in human and murine cells. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 80 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Ramo cell lysate. Western Blotting Analysis: 4 µg/mL of this antibody detected MRE11 in 10 µg of Ramo cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |