| Replacement Information | |
|---|---|
| Replacement Information | 04-239 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1328 |
| Description | Anti-INK4a (p16) Antibody, clone 13H4.1 |
| Alternate Names |
|
| Background Information | Cyclin-dependent kinase inhibitor 2A (UniProt P42771; also known as CDK4I, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, MTS-1, p16-INK4, p16-INK4a, p16INK4A) is encoded by the CDKN2A (also known as CDKN2, MTS1) gene (Gene ID 1029) in human. The INK and CIP/KIP families of cyclin dependent kinase inhibitors (CDKis) protect cells from oncogenic signals-initiated cellular transformation. INK4a and INK4b (encoded by the CDKN2A and CDKN2B gene, respectively) constitute the INK4 subfamily of CDKis. INK4a and INK4b are induced by oncogenic Ras and are involved in cell cycle G1 arrest and cellular senescence. Inactivation of CDKN2A and CDKN2B are found in a wide variety of human malignancies and murine tumor models. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-INK4a (p16), clone 13H4.1 Antibody, Cat. No. MABE1451, is a highly specific mouse monoclonal antibody that targets INK4a (p16) and has been tested in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p16INK4a in human kidney and small intestine tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human p16INK4a internal fragment. |
| Clone | 13H4.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 13H4.1 epitope lies within a region present in p16INK4a and the spliced isoforms 2 and 5 (UniProt P42771-1, P42771-2, and P42771-4 respectively), but absent from the spliced isoform 3 (p12; UniProt P42771-3), p14ARF/p19ARF or smARF (UniProt Q8N726-1 and Q8N726-2). |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified. |
| UniProt Number |
|
| Molecular Weight | ~16 kDa observed. 16.53 kDa (isoform 1; p16INK4a), 11.22 kDa (isoform 2), and 17.88 (isoform 5; p16gamma) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected p16INK4a in 10 µg of HEK293 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |