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Millipore Anti-Ink4a (P16), Clone 13h4.1 Antibody, Cat. No. Mabe1451, Is A Highly Specific Mouse Monoclonal Antibody That Targets Ink4a (P16) And Has Been Tested In Immunocytochemistry - Mill (Additional S&H or Hazmat Fees May Apply)
Cyclin-dependent kinase inhibitor 2A (UniProt P42771; also known as CDK4I, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, MTS-1, p16-INK4, p16-INK4a, p16INK4A) is encoded by the CDKN2A (also known as CDKN2, MTS1) gene (Gene ID 1029) in human. The INK and CIP/KIP families of cyclin dependent kinase inhibitors (CDKis) protect cells from oncogenic signals-initiated cellular transformation. INK4a and INK4b (encoded by the CDKN2A and CDKN2B gene, respectively) constitute the INK4 subfamily of CDKis. INK4a and INK4b are induced by oncogenic Ras and are involved in cell cycle G1 arrest and cellular senescence. Inactivation of CDKN2A and CDKN2B are found in a wide variety of human malignancies and murine tumor models.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-INK4a (p16), clone 13H4.1 Antibody, Cat. No. MABE1451, is a highly specific mouse monoclonal antibody that targets INK4a (p16) and has been tested in Immunocytochemistry and Western Blotting.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p16INK4a in human kidney and small intestine tissue sections.
Biological Information
Immunogen
GST-tagged recombinant human p16INK4a internal fragment.
Clone
13H4.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 13H4.1 epitope lies within a region present in p16INK4a and the spliced isoforms 2 and 5 (UniProt P42771-1, P42771-2, and P42771-4 respectively), but absent from the spliced isoform 3 (p12; UniProt P42771-3), p14ARF/p19ARF or smARF (UniProt Q8N726-1 and Q8N726-2).
Isotype
IgG2bκ
Species Reactivity
Human
Species Reactivity Note
Human.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000068
Gene Symbol
CDKN2A
CDKN2
MTS1
Purification Method
Protein G purified.
UniProt Number
P42771
Molecular Weight
~16 kDa observed. 16.53 kDa (isoform 1; p16INK4a), 11.22 kDa (isoform 2), and 17.88 (isoform 5; p16gamma) calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected p16INK4a in 10 µg of HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
