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Millipore This Anti-Nuf2 Antibody, Clone 28-37 Is Validated For Use In Western Blotting, Immunocytochemistry, Immunoprecipitation For The Detection Of Nuf2. - Mill (Additional S&H or Hazmat Fees May Apply)
Kinetochore protein Nuf2 (UniProt Q9BZD4; also known as Cell division cycle-associated protein 1, hNuf2, hNuf2R, hsNuf2) is encoded by the NUF2 (also known as CDCA1, NUF2R) gene (Gene ID 83540) in human. Nuf2 is a kinetochore complex component that plays an important role in stabilizing microtubule attachment. Nuf2 knockdown results in kinetochore attachment defects and eventual death of mitotic cells. Nuf2 binds and stabilizes CENP-E in the kinetochore, and both proteins are essential for proper chromosome segregation during mitosis. Nuf2 is also shown to complex with Hec1, and the Nuf2-Hec1 complex is essential for organizing microtubule attachment sites. Nuf2 upregulation is reported in various cancers, and Nuf2 knockdown results in significant cell growth delay and apoptosis among non-small cell lung, colorectal, gastric, and ovarian cancer cell lines.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Nuf2 Antibody, clone 28-37 is validated for use in Western Blotting, Immunocytochemistry, Immunoprecipitation for the detection of Nuf2.
Key Applications
Western Blotting
Immunocytochemistry
Immunoprecipitation
Application Notes
Immunocytochemistry Analysis: A representative lot detected Nuf2 in Nocodazole-treated HeLa cells by immunofluorescence microscopy (Stucke, V.M., et al. (2004). Chromosoma. 113(1):1-15). Immunoprecipitation Analysis: A representative lot immunoprecipitated Nuf2 (Stucke, V.M., et al. (2004). Chromosoma. 113(1):1-15).
Biological Information
Immunogen
His-tagged full-length human Nuf2.
Clone
28-37
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
The specificity of clone 28-37 was confirmed by immunoblotting, immunofluorescence microscopy, and immunoprecipitation (Stucke, V.M., et al. (2004). Chromosoma. 113(1):1-15).
Isotype
IgG1κ
Species Reactivity
Human
Species Reactivity Note
Human.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_113611
Gene Symbol
CDCA1
NUF2
NUF2R
Purification Method
Protein G purified.
UniProt Number
Q9BZD4
Molecular Weight
~54 kDa observed. 54.30 kDa calculated.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell acid extract.
Western Blotting Analysis: A 1:125 dilution of this antibody detected Nuf2 in 10 µg of HeLa cell acid extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
