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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1531 |
| Replaces | 05-905 |
| Description | Anti-Nuf2 Antibody, clone 28-37 |
| Alternate Names |
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| Background Information | Kinetochore protein Nuf2 (UniProt Q9BZD4; also known as Cell division cycle-associated protein 1, hNuf2, hNuf2R, hsNuf2) is encoded by the NUF2 (also known as CDCA1, NUF2R) gene (Gene ID 83540) in human. Nuf2 is a kinetochore complex component that plays an important role in stabilizing microtubule attachment. Nuf2 knockdown results in kinetochore attachment defects and eventual death of mitotic cells. Nuf2 binds and stabilizes CENP-E in the kinetochore, and both proteins are essential for proper chromosome segregation during mitosis. Nuf2 is also shown to complex with Hec1, and the Nuf2-Hec1 complex is essential for organizing microtubule attachment sites. Nuf2 upregulation is reported in various cancers, and Nuf2 knockdown results in significant cell growth delay and apoptosis among non-small cell lung, colorectal, gastric, and ovarian cancer cell lines. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Nuf2 Antibody, clone 28-37 is validated for use in Western Blotting, Immunocytochemistry, Immunoprecipitation for the detection of Nuf2. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected Nuf2 in Nocodazole-treated HeLa cells by immunofluorescence microscopy (Stucke, V.M., et Al. (2004). Chromosoma. 113(1):1-15). Immunoprecipitation Analysis: A representative lot immunoprecipitated Nuf2 (Stucke, V.M., et Al. (2004). Chromosoma. 113(1):1-15). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full-length human Nuf2. |
| Clone | 28-37 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | The specificity of clone 28-37 was confirmed by immunoblotting, immunofluorescence microscopy, and immunoprecipitation (Stucke, V.M., et Al. (2004). Chromosoma. 113(1):1-15). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified. |
| UniProt Number |
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| Molecular Weight | ~54 kDa observed. 54.30 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell acid extract. Western Blotting Analysis: A 1:125 dilution of this antibody detected Nuf2 in 10 µg of HeLa cell acid extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |