Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ELISA | Rb | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1782-25UL |
| Description | Anti-phospho-E2F1 (Ser375) Antibody, clone 71-10 |
| Alternate Names |
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| Background Information | Transcription factor E2F1 (UniProt: Q01094; also known as E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1) is encoded by the E2F1 (also known as RBBP3) gene (Gene ID: 1869) in human. E2F-1 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5-TTTC[CG]CGC-3 found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. It is a component of the DRTF1/E2F transcription factor complex and forms heterodimers with DP family members. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1 complex is shown to bind specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase and detaches from the DRTF1/E2F complex that renders E2F transcriptionally active. E2F-1 can be phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It is also phosphorylated at serine 364 by CHEK2, which stabilizes it upon DNA damage and regulates its effect on transcription and apoptosis. Acetylation of E2F-1 by P/CAF/KAT2B stimulates its DNA-binding. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-phospho-E2F1 (Ser375), clone 71-10, Cat. No. MABE1782, is a highly specific rabbit monoclonal antibody that targets Transcription factor E2F1 phosphorylated on Serine 375 and has been tested in ELISA and Western Blotting. |
| Key Applications |
|
| Application Notes | ELISA Analysis: A representative lot detected phospho-E2F1 (Ser375) in ELISA applications (Dale, T., et. al. (2015). Nat Chem Biol. 11(12):973-980). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 17 amino acids surrounding phosphoserine 375 from the C-terminal region of human transcription factor E2F1. |
| Clone | 71-10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Clone 71-10 is a rabbit monoclonal antibody that specifically detects human E2F1 phosphorylated on Serine 375. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~80 kDa observed ( GST fusion protein); 46.92 kDa calculated (without GST). Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cells transfected with pcDNA3.1 empty vector, HEK 293 cells transfected with E2F1 wild type-GST, HEK 293 cells transfected with E2F1 wild type-GST with alanine substituted for serine at 375. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected phospho-E2F1 (Ser375) in HEK293 cells transfected with E2F1 wild type-GST,, but not in HEK 293 cells transfected with E2F1 wild type-GST with alanine substituted for serine at 375. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |