Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1795-100UL |
| Description | Anti-PP4C Antibody, clone 7B5 |
| Alternate Names |
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| Background Information | Serine/threonine-protein phosphatase 4 catalytic subunit (UniProt: P60510; also known as EC: 3.1.3.16, PP4C, Pp4, Protein phosphatase X, PP-X) is encoded by the PPP4C (also known as PPP4, PPX) gene (Gene ID: 5531) in human. Protein phosphatase 4 (PP4), a ubiquitous serine/threonine phosphatase, is involved in the regulation of many cellular processes. It functions independently of protein phosphatase 2A (PP2A). Mammalian PP4 contains two regulatory subunits (R1 and R2) that interact with the catalytic subunit (PP4C)) and control its activity. Methylation at the C-terminal Leucine 307 is critical for interactions with regulatory subunits. PP4C-R2 complexes are reported to be involved in organelle assembly, maturation of the centrosome, apoptosis, DNA repair, and spliceosomal assembly. It is also reported to play a role in cell migration and in activation of c-Jun N-terminal kinase and NF-kappa B. PP4C has multiple metal ion binding sites and can bind two manganese ions per subunit. (Ref.: Cohen, PTW., et al. (2005). FEBS Lett. 579(15); 3278-3286). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-PP4C, clone 7B5, Cat. No. MABE1795, is a highly specific mouse monoclonal antibody that targets Serine/threonine-protein phosphatase 4 catalytic subunit and has been tested for use in Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected PP4C in lysate from NIH3T3 mouse fibroblasts ectopically expressing N-terminally tagged human PP4 catalytic subunit (PPP4C).(Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide coreesponding to 17 amino acids from the C-terminal region of human Serine/threonine-protein phosphatase 4 catalytic subunit (PP4C). |
| Clone | 7B5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 7B5 specifically detects human Serine/threonine-protein phosphatase 4 catalytic subunit (PP4C). It targets an epitope within 17 amino acids from the C-terminal region. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Rat, Rabbit, Bovine based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 35.08 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Isotype testing: Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |