| Description | |
|---|---|
| Catalogue Number | MABE1807-25UG |
| Description | Anti-NuMA1 Antibody, clone AD6-1 |
| Alternate Names |
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| Background Information | Nuclear mitotic apparatus protein 1 (UniProt: Q14980; also known as NuMA protein, Nuclear matrix protein-22, NMP-22, SP-H antigen) is encoded by the NUMA1 (also known as NMP22, NUMA) gene (Gene ID: 4926) in human. NMP-22 is a homodimeric nuclear protein that is highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role and occupies the majority if the nuclear volume. It is required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes. It is also considered to be essential or proper alignment of the mitotic spindle during asymmetric cell divisions. NMP-22 resides in the nuclear matrix during interphase and then dissociates from condensing chromosomes during early prophase and relocates to the spindle poles via dynein/dynamin association and it remains there until the anaphase onset. As mitosis progresses it re-associates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. NMP-22 is phosphorylated in the C-terminal tail during mitosis, most likely by CDK1 and phosphorylation increases its solubility and promotes association with dynein and subsequent translocation to the spindle poles. O-glycosylation during cytokinesis at sites identical or close to phosphorylation sites interferes with the phosphorylation status of NMP-22. |
| Product Information | |
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| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-NuMA1, clone AD6-1 Antibody, Cat. No. MABE1807-25UG, is a highly specific mouse monoclonal antibody, that targets NUMA1 and has been tested in Western Blotting and Immunocytochemistry. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected NUMA1 in 10 µg of NIH/3T3 cell lysate. Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected NUMA1 in HepG2 cells. Immunocytochemistry Analysis: A representative lot detected NUMA1 in Immunocytochemistry applications (Carminati, M., et. Al. (2016). Nat Struct Mol Biol. 23(2):155-63; Gallini, S., et. Al. (2016). Curr Biol. 26(4):458-69). Western Blotting Analysis: A representative lot detected NUMA1 in Western Blotting applications (Carminati, M., et. Al. (2016). Nat Struct Mol Biol. 23(2):155-63; Gallini, S., et. Al. (2016). Curr Biol. 26(4):458-69). |
| Biological Information | |
|---|---|
| Immunogen | A recombinant fragment corresponding to 181 amino acids from the C-terminal region of human Nuclear mitotic apparatus protein 1 (NuMA1). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone AD6-1 detects NuMA1 in human and mouse cells. It targets an epitope within 181 amino acids from the C-terminal region. |
| Isotype | IgG1 |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 250 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected NUMA1 in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |