Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Bact | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1823-100UG |
| Description | Anti-AsCpf1 Antibody, clone 3D3-F7 |
| Alternate Names |
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| Background Information | CRISPR-associated endonuclease Cas12a (UniProt: U2UMQ6; also known as EC: 3.1.27.2, AsCpf1, CRISPR-associated endonuclease Cpf1) I encoded by the Cas12a (also known as cpf1) gene in Acidaminococcus species. CRISPR is an adaptive immune system that provides protection against mobile genetic elements, such as viruses, transposable elements, and conjugative plasmids. CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. They are transcribed and processed into CRISPR RNA (crRNA). Cas12a, an endonuclease from the type V-A CRISPR system, has several unique characteristics when compared to Cas9 system that increase the utility of CRISPR-based gene editing. Cas12a mediated cleavage utilizes a single and short CRISPR RNA) without requiring trans-activating crRNA. Cas12a recognize a 5 T-rich protospacer adjacent motif (PAM, TTTN for this specific enzyme), unlike Cas9 enzymes which recognize 3 g-rich PAMs, thus this enzyme increases the possibilities for genome editing. The endonuclease activity of Cas12a results in staggered 4-base 5 overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-AsCpf1, clone 3D3-F7, Cat. No. MABE1823, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cpf1 and has been tested in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AsCpf1 in HEK293 cells expressing HA-tagged AsCpf1 (Courtesy of Stefan Schuchner, pH.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant fragment corresponding to 197 amino acids from the C-terminal region of Acidaminococcus Cas12a. |
| Clone | 3D3-F7 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3D3-F7 specifically detects CRISPR-associated endonuclease Cas12a. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Acidaminococcus. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~151 kDa observed; , 151.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cells expressing HA-tagged AsCpf1. Western Blotting Analysis: 1 µg/mL of this antibody detected AsCpf1 in lysate from HEK293 cells expressing HA-tagged AsCpf1. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |