Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Bact | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1824-25UG |
| Description | Anti-LbCpf1 Antibody, clone 2D5-6G11 |
| Alternate Names |
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| Background Information | CRISPR-associated endonuclease Cas12a (UniProt: A0A182DWE3; also known as CRISPR-associated endonuclease Cpf1; Lbcpf1) is a single crRNA-guided endonuclease of the class 2 CRISPR-Cas system that lacks tracrRNA and undergoes large conformational changes upon crRNA binding. Cpf1 has several features that can distinguish it from Cas9. Unlike Cas9 that uses dual-guide RNAs, Cpf1 is guided by a crRNA. It recognizes a T-rich PAM (T-rich protospacer adjacent motif), whereas Cas9 favors a g-rich PAM and Cpf1 generates staggered ends at its PAM-distal region, but Cas9 creates blunt ends at its PAM-proximal region. Cpf1 displays higher targeting specificity in mammalian cells when compared to Cas9. It has been shown that depending on the PAM sequences, LbCpf1 can undergo conformational changes to form altered interactions with the PAM-containing DNA duplexes, hence achieving the relaxed PAM recognition. (Ref.: Zetsche, B., et Al. (2015). Cell 163(3); 759-77; Yamano, T., et Al. (2017). Mol. Cell 67(4); 633-645; Gao, P., et Al. (2016). Cell Res. 26(8); 901-913). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-LbCpf1, clone 2D5-6G11 Antibody, Cat. No. MABE1824, is a highly specific mouse monoclonal antibody that targets LbCpf1 and has been tested for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: Various dilutions from a representative lot detected LbCpf1 in lysates from HEK293 cells expressing HA-tagged LbCpf1 (Courtesy of Stefan Schuchner, pH.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant fragment from the N-terminal region of Lachnospiracea bacteria. |
| Epitope | N-terminus |
| Clone | 2D5-6G11 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 2D5-6G11 specifically detects CRISPR-associated endonuclease Cas12a (LbCpf1). |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Lachnospiracea. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein g purified |
| Molecular Weight | ~144 kDa observed; 143.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cells expressing HA-tagged LbCpf1. Western Blotting Analysis: 1 µg/mL of this antibody detected LbCpf1 in lysate from HEK293 cells expressing HA-tagged LbCpf1. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |