Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ChIP, DB, IP, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1828-25UL |
| Description | Anti-beta-Catenin Antibody, clone mAb-Cat1 |
| Alternate Names |
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| Background Information | Catenin beta-1 (UniProt: P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, OK/SW-cl.35, PRO2286) gene (Gene ID: 1499) in human. Catenin beta-1 is a key downstream component of the canonical Wnt signaling pathway that participates in intercellular adhesion and Wnt-mediated transcriptional activation. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK-3 beta that promotes phosphorylation on N-terminal serine and threonine residues that leads to its subsequent degradation by the proteasome. In the presence of Wnt ligand, it is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, which leads to activation of Wnt responsive genes. O-glycosylation at serine 23 is reported to decreases its nuclear localization and transcriptional activity. Wnt/beta-catenin signaling is shown to be essential for embryonic stem cell (ESC) pluripotency and differentiation. In ESC beta-catenin is trimethylated on Lysine 49 by Ezh2 methyltransferase and this trimethylated form represses neuronal differentiation genes Sox1 and Sox3. Mutations in CTNNB1 gene are shown to cause colorectal cancers, medulloblastoma, and ovarian cancers. (Ref.: Hoffmeyer, K., et Al. (2017). Cell Reports 18(12); 2815-2824. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-beta-Catenin, clone mAb-Cat1, Cat. No. MABE1828, is a highly specific rat monoclonal antibody that targets beta-catenin 1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), DOT Blot, Immunoprecipitation, and Western Blottiing. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected beta-Catenin in Western Blotting applications (Hoffmeyer, K., et. Al. (2017). Cell Rep. 18(12):2815-2824). Immunoprecipitation Analysis: A representative lot immunoprecipitated beta-Catenin in Immunoprecipitation applications (Hoffmeyer, K., et. Al. (2017). Cell Rep. 18(12):2815-2824). DOT Blot Analysis: A representative lot detected beta-Catenin in DOT Blot applications (Hoffmeyer, K., et. Al. (2017). Cell Rep. 18(12):2815-2824). Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated beta-Catenin in Chromatin Immunoprecipitation applications (Hoffmeyer, K., et. Al. (2017). Cell Rep. 18(12):2815-2824). |
| Biological Information | |
|---|---|
| Immunogen | BSA-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human beta-catenin 1. |
| Clone | mAb-Cat1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | This rat monoclonal antibody detects beta-catenin 1 unmodified and methylated or acetylated on lysine 49. It targets an epitope with in 19 amino acids from the N-terminal region. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Bovine and Mouse based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~100 kDa observed; 85.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in SW 480 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected beta-Catenin in SW 480 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |