Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ChIP, FC, ICC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1831-25UG |
| Description | Anti-CSB (ERCC6) Antibody, clone ICSB-3H8 |
| Alternate Names |
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| Background Information | DNA excision repair protein ERCC-6 (UniProt: Q03468; also known as ATP-dependent helicase ERCC6, Cockayne syndrome protein CSB) is encoded by the ERCC6 (also known as CSB) gene (Gene ID: 2074) in human. CSB is a homodimeric nuclear protein that belongs to the SwItch/ Sucrose nonfermentable family of DNA-dependent ATPases and contains seven characteristic helicase motifs. It is a part of a complex that contains RNA pol I, TFIIH, and XPG, all of which are active in transcription and/or DNA repair. Hence, it is an essential factor involved in transcription-coupled nucleotide excision repair, which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA. It is shown to be essential for transcription-coupled repair complex formation. It recruits the CSA complex (DCX(ERCC8) complex), nucleotide excision repair proteins and EP300 to the at sites of RNA polymerase II-blocking lesions. Mutations in CSB (ERCC6) gene are known to cause age related macular degeneration and Cockayne syndrome B that is characterized by delayed neural development and severe progressive neurologic degeneration and mental retardation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-CSB (ERCC6), clone ICSB-3H8, Cat. No. MABE1831, is a highly specific mouse monoclonal antibody that targets DNA excision repair protein ERCC-6 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected CSB (ERCC6) in Immunocytochemistry applications (Bradsher, J., et. Al. (2002). Mol Cell. 10(4):819-29). Immunoprecipitation Analysis: A representative lot immunoprecipitated CSB (ERCC6) in Immunoprecipitation applications (Bradsher, J., et. Al. (2002). Mol Cell. 10(4):819-29). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CSB (ERCC6) in Chromatin Immunoprecipitation applications (Kristensen, U., et. Al. (2013). Proc Natl Acad Sci USA. 110(25):E2261-70; Velez-Cruz, R., et. Al. (2012). Proc Natl Acad Sci USA. 110(3):E212-20). Western Blotting Analysis: A representative lot detected CSB (ERCC6) in Western Blotting applications (Bradsher, J., et. Al. (2002). Mol Cell. 10(4):819-29). |
| Biological Information | |
|---|---|
| Immunogen | Ovalbumin-conjugated linear Peptide corresponding to 22 amino acids from the C-terminal region of human DNA excision repair protein ERCC-6. |
| Clone | 1CSB-3H8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 1CSB-3H8 specifically detects DNA excision repair protein ERCC-6 in human cells. It targets an epitope with in 22 amino acids from the C-terminal region. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 168.42 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in A431 cells. Flow Cytometry Analysis: 1 µg of this antibody detected CSB (ERCC6) in one million A431 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |