| Description | |
|---|---|
| Catalogue Number | MABE1886-100UG |
| Description | Anti-BMAL1 (ARNTL) Antibody, clone B1BH2 |
| Alternate Names |
|
| Background Information | Aryl hydrocarbon receptor nuclear translocator-like protein 1 (UniProt: Q9I8T7; also known as BMAL1b, Brain and muscle ARNT-like 1, cBMAL1) is encoded by the ARNTL (also known as BMAL1) gene (Gene ID: 374115) in chicken. BMAL1 is expressed in pineal gland and retina and displays maximum levels between ZT13 and ZT16 during light/dark cycle. BMAL1 is a transcriptional activator that forms a core component of the circadian clock. These components include ARNTL/BMAL1, CLOCK, NPAS2, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1, and CRY2. CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2 form the positive limb of the feedback loop. They act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes that are involved in key metabolic processes. PER1/2/3 and CRY1/2, act as transcriptional repressors and form the negative limb of the feedback loop. They interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity. BMLA1 is highly expressed in the adult brain, skeletal muscle, and heart. It shuttles between the nucleus and the cytoplasm and this nucleocytoplasmic shuttling is essential for the nuclear accumulation of CLOCK, target gene transcription, and the degradation of the CLOCK-ARNTL/BMAL1 heterodimer. BMLA1 is reported to be phosphorylated upon dimerization with CLOCK and this phosphorylation enhances its transcriptional activity and reduces the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. BMAL1 null-mice are reported to have reproductive problems, reduced locomotor activity, and premature aging. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-BMAL1 (ARNTL), clone B1BH2, Cat. No. MABE1886, is a highly specific mouse monoclonal antibody that targets Brain and muscle ARNT-like 1 (BMAL1) and has been tested for use in Chromatin Immunoprecipitation (ChIP) and Western Blotting. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected BMAL1 (ARNTL) in Chromatin Immunoprecipitation applications (Yoshitane, H., et. Al. (2014). Mol Cell Biol. 34(10):1776-87). Western Blotting Analysis: A representative lot detected BMAL1 (ARNTL) in Western Blotting applications (Yoshitane, H., et. Al. (2009). Mol Cell Biol. 29(13):3675-86; Yoshitane, H., et. Al. (2012). EMBO Rep. 13(5):455-61). |
| Biological Information | |
|---|---|
| Immunogen | MBP-conjugated recombinant fragment corresponding to 56 amino acids from the N-terminal region of chicken BMAL1. |
| Clone | B1BH2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone B1BH2 specifically detects Brain and muscle ARNT-like 1 (BMAL1). It targets an epitope within 56 amino acids from the N-terminal region. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Predicted to react with Rat, Human, Chicken based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein g purified |
| UniProt Number |
|
| Molecular Weight | ~75 kDa observed; 69.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in NIH/3T3 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected BMAL1 (ARNTL) in NIH/3T3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |