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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE270 |
| Description | Anti-phospho STAT6 (Tyr641) Antibody, clone 19E2.1 |
| Alternate Names |
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| Background Information | Signal transducer and activator of transcription 6 (STAT6) transduces signals from extracellular cytokines during adaptive immunity and has recently been found to be important in the innate immune response to viral infection. STAT6 is also involved in the attenuation of the Th1 and Th17 in the nephritogenic immune response. The consequence of STAT6 activity has been associated with the pathogenesis of autoimmune disease and has been found to be actively imported to the nucleus. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho STAT6 (Tyr641) Antibody, clone 19E2.1 is a mouse monoclonal antibody for detection of phospho STAT6 (Tyr641) also known as Signal transducer & activator of transcription 6, IL-4 Stat & has been validated in WB, FC. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 2 µg/mL from a representative lot detected STAT6 in human peripheral blood leukocytes. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human STAT6 phosphorylated at (Tyr641). |
| Epitope | Phosphorylated (Tyr641) |
| Clone | 19E2.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes STAT6 phosphorylated at (Tyr641). |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]. |
| Gene Symbol |
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| Modifications |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling. SUBUNIT STRUCTURE: Forms a homodimer or a heterodimer with a related family member By similarity. Interacts with NCOA1 via its C-terminal LXXLL motif. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation. PTM: Tyrosine phosphorylated following stimulation by IL-4 and IL-3 (By similarity). SEQUENCE SIMILARITIES: Belongs to the transcription factor STAT family. Contains 1 SH2 domain. |
| Molecular Weight | ~165 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in NIH/3T3 cell lysates treated and untreated with PDGF. Western Blot Analysis: 0.5 µg/mL of this antibody detected STAT6 on 10 µg of PDGF untreated and treated NIH/3T3 cell lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |